L-半胱氨酸/普鲁士蓝复合修饰玻碳电极的制备及电化学性能  

Preparation and Electrochemical Properties of Glassy Carbon Electrode Modified by L-Cysteine/Prussian Blue

作　　者：何家洪[1,2,3] 张胜涛[1] 邢少华 宋仲容[3] 

机构地区：[1]重庆大学化学化工学院,重庆400030 [2]中船重工七二五所青岛分部海洋腐蚀与防护重点实验室,青岛266101 [3]重庆文理学院材料与化工学院,永川402160

出　　处：《分析化学》2013年第10期1561-1565,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.41101223);重庆市教育委员会资助项目(Nos.KJ111206;KJ1312105);重庆文理学院重点项目(No.Z2011HH16);重庆文理学院学生科研项目(No.201110)资助

摘　　要：通过电聚合方法和脉冲沉积技术将普鲁士蓝（PB）与，L-半胱氨酸（L—Cys）修饰在玻碳电极（GCE）表面，制得复合膜化学修饰电极（L—Cys／PB／GCE／CME），利用循环伏安法和计时安培法研究了对苯二酚在L—Cys／PB／GCE／CME上的电化学特征。结果表明，在0．1mol／LPBS（pH7．0）缓冲溶液中，L—Cys／PB／GCE／CME对对苯二酚的电化学氧化具有明显的催化作用，氧化峰电流相对于在裸玻碳电极上增加了5倍。在最佳实验条件下，对苯二酚浓度在0．18～120μmol／L范围内，修饰电极的电流响应与对苯二酚浓度呈线性关系，其相关系数为0．9962，检出限（S／N=3）为0．065μmoX／L。本研究制备的对苯二酚传感器具有较好的重复性、重现性、选择性与稳定性，用于实际水样中对苯二酚的测定，结果满意。A glassy carbon electrode （GCE） modified by Prussian blue （PB） and L-Cysteine （L-Cys） composite film was prepared by the method of electro-polymerization and pulse electrodeposition. The electrochemical performance of PB/L-Cys/GCE/CME for detection of hydroquinone was investigated by cyclic vohammetry and chronoamperometry. The results showed that in 0. 1 mol/L PBS （pH 7.0） buffer, PB/L- Cys/GCE/CME exhibited excellent catalytic and enhancement effect on the electrochemical oxidation of hydroquinone, and the oxidation peak current increased ca. 5 times as compared with that at bare GCE. Under the optimal conditions, hydroquinone concentration was linear with peak current in the range of 0.18- 120 μmol/L with a correlation coefficient of 0. 9962, and the detection limit was 0. 065 μmol/L （ S/N= 3 ）. The proposed hydroquinone biosensor has good repeatability, reproducibility, selectivity and stability, and can be applied to determine the hydroquinone content in water sample.

关 键 词：普鲁士蓝 L-半胱氨酸 修饰电极 对苯二酚 

分 类 号：O657.1[理学—分析化学]