人皮肤鳞状细胞癌体外三维模型的建立及高侵袭性鳞癌细胞的生物学评价  

The establishment of the three-dimensional culture model of the human skin squamous-celled carcinoma and their biological evaluation of the high-invasive squamous carcinoma cells

作　　者：杨晓鲲[1] 杨亚东[1] 杨桂红[1] 唐书谦[1] 伍津津[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所皮肤科,重庆400042

出　　处：《重庆医学》2013年第29期3465-3467,3471,共4页Chongqing medicine

基　　金：国家高技术研究发展计划(863计划)资助项目(2012AA020504-4);国家自然科学基金资助项目(31170924)

摘　　要：目的建立人皮肤鳞癌细胞体外三维培养模型,分离高侵袭性细胞亚群,并比较其生物学特性。方法利用复方壳多糖组织工程皮肤,成功建立人皮肤鳞癌细胞体外三维培养模型,消化分离得到高侵袭性鳞癌细胞亚群,比较肉桂醛和干扰素作用后对不同侵袭特性细胞亚群的增殖抑制效果,同时比较不同侵袭特性细胞亚群在裸鼠中成瘤效果的不同。结果不同浓度的肉桂醛和干扰素对皮肤鳞癌细胞的增殖抑制差异有统计学意义(P<0.01),不同侵袭特性的皮肤鳞癌细胞亚群之间的增殖抑制差异也有统计学意义(P<0.01),另外,两种不同侵袭特性的细胞亚群的成瘤特性差异有统计学意义(P<0.01)。结论成功建立人皮肤鳞癌体外三维培养模型,同时运用此种三维培养模型能够筛选得到侵袭特性更强的细胞亚群。Objective To establish the three-dimensional culture model of the human skin squamous-celled carcinoma ,and isolate the highly invasive cell subsets ,and compare their biological characteristics .Methods By using the composite chitosan tissue engi-neering skin ,the three-dimensional culture model of the human skin squamous-celled carcinoma was successfully established , through digestion ,we divided and got the high-invasive squamous carcinoma cells subsets .After that ,the different invasive proper-ties of subpopulations of cells in nude mice tumor effects were compared .Results Different concentrations of cinnamaldehyde and interferon on skin squamous-celled carcinoma cell proliferation inhibition had statistical significance （P〈0 .01） ,the different invasive characteristics of skin squamous cells carcinoma between subpopulations of cells proliferation inhibition also was statistically signifi-cant（P〈0 .01） ,and the two different invasive properties of subpopulations of cells in the tumor characteristics also had statistical significance（P〈0 .01） .Conclusion We established the three-dimensional culture model of the human skin squamous-celled carcino-ma successfully ,by using this model ,we could screen the higher invasive cells subsets .

关 键 词：三维培养模型 组织工程皮肤 癌 鳞状细胞 高侵袭性 

分 类 号：R739.5[医药卫生—肿瘤]