大鼠蛛网膜下腔出血后早期ADAMTS-4的表达与血脑屏障通透性的相关性研究  

Correlation study on the ADAMTS-4 in early brain injury after experimental subarachnoid hemorrhage and blood-brain barrier permeability in rats

作　　者：岳四海[1] 刘献志[1] 张宏凤[1] 

机构地区：[1]郑州大学第一附属医院神经外科/郑州市神经病学重点实验室,450000

出　　处：《重庆医学》2013年第29期3518-3520,共3页Chongqing medicine

摘　　要：目的通过建立大鼠蛛网膜下腔出血(subarachnoid hemorrhage,SAH)的动物模型,对ADAMTS-4在SAH后早期脑损伤中的作用进行初步研究。方法成年雄性SD大鼠72只,分对照组和SAH组,通过检测大鼠海马ADAMTS-4的表达及脑内伊文氏蓝(Evans Blue,EB)的浓度,并通过股静脉注射ADAMTS-4抑制剂-α2巨球蛋白进行干预等,探讨ADAMTS-4的表达与BBB通透性的相关性。结果 SAH后24hADAMTS-4mRNA及蛋白表达明显升高,脑内EB浓度于出血后12h明显升高,ADAMTS-4蛋白表达与脑内EB浓度成正相关(r=0.917,P<0.05)。经α2巨球蛋白干预后,SAH后48hADAMTS-4蛋白表达较非干预组明显降低(P<0.05),且干预组脑内EB浓度较非干预组亦有显著减少(P<0.05)。结论 ADAMTS-4可能参与了SAH后早期脑损伤的病理过程。Objective To establish the rat model of experimental subarachnoid hemorrhage （SAH） and to detect the expression of ADAMTS-4 in early brain injury（EBI） after SAH in rats .Methods Totally 72 adult male SD rats were randomly divided into 2 groups ,the control group and SAH group .The expression of ADAMTS-4 and the content of brain Evans Blue（EB） were detected . The ADAMTS-4 inhibitor α2-macroglobulin was used to study the relationship between the expression of ADAMTS-4 and blood-brain barrier permeability .Results The expression of the mRNA and protein of ADAMTS-4 increased dramatically at 24 h and the contents of EB increased progressively at 12 h after SAH .The protein expression of ADAMTS-4 and the content of brain EB was positive correlated（r=0 .917 ,P〈0 .05） .The expression of ADAMTS-4 was inhibited by α2-macroglobulin（P〈0 .05） and the con-tents of EB decreased dramatically （P〈0 .05） .Conclusion ADAMTS-4 may be involved in the pathological progression of early brain injury after SAH .

关 键 词：ADAMTS-4 蛛网膜下腔出血 脑损伤 

分 类 号：R743.35[医药卫生—神经病学与精神病学]