改良后高脂饮食所致大鼠非酒精性脂肪性肝病模型的建立  

Establishment of Non-alcoholic Fatty Liver Disease Rat Models Induced by Improved High-fat Diet

作　　者：李玲[1] 孙学华[1] 李曼[1] 周振华[1] 高月求[1] 

机构地区：[1]上海中医药大学附属曙光医院肝炎科

出　　处：《山西中医学院学报》2013年第3期8-10,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2012ZX10005004-002;2012ZX-10005010-002-003);国家自然基金(81072792;81102570;81202662);国家中医药管理局中医肝胆病重点学科(2010sh);上海市自然科学基金(10ZR1430900);上海市科委临床重点项目(11411951600);上海市教育委员会重点学科(第五期)建设资助项目(J50307);上海高校创新团队项目(第一期);上海市教委预算内科研项目(2010JW43);上海中医药大学优秀团队培养计划;上海市中医药事业发展三年行动计划项目(ZYSNXD-CC-ZDYJ015)

摘　　要：目的:探讨非酒精性脂肪性肝病动物模型的一种快速的建立方法。方法:将30只雄性大鼠随机分为正常组和模型组,分别喂养普通基础饲料和改良后高脂饲料10 w。比较两组大鼠血清ALT、AST、DBIL、HDL-C、LDL-C、TC和肝组织匀浆GGT、TG、GSH含量的变化及肝脏组织学变化。结果:与正常组比较,第10周时模型组大鼠血清ALT、AST、DBIL、HDL-C、LDL-C、TC、TC/HDL-C均明显升高(P<0.05);肝组织匀浆GGT、TG含量升高,GSH含量降低(P<0.05)。正常组大鼠肝组织未见明显脂肪变性及炎症,模型组大鼠在小泡性脂肪变性的基础上伴随淋巴细胞浸润和灶状坏死。结论:改良后高脂饲料可加快非酒精性脂肪性肝病的造模进程,值得推广。Objective:To approach a method of establishing quickly non-alcoholic fatty liver disease animal models.Method:30 male rats were randomly divided into normal group and model group.Rats in two groups were respectively fed normal diet and improved high-fat diet for 10 weeks.Serum aminotransferase value,lipids level and contents of GGT、TG、GSH in liver homogenate were examined and pathological changes of liver tissue were observed.Result:Compared with normal group,at the tenth week,contents of ALT、AST、DBIL、HDL-C、LDL-C、TC、TC/HDL-C in serum were significantly increased(P<0.05)in model group;levels of GGT、TG in liver homogenate were significantly increased(P<0.05),GSH was significantly decreased in model group(P<0.05).On the basis of the small steatosis,there's a large number of lymphocytic infiltration and focal necrosis in liver tissue of rats in model group.There′s no obvious fatty degeneration and inflammation in liver tissue of rats in normal group.Conclusion:Improved high-fat diet could speed up the modeling process of non-alcoholic fatty liver disease models.

关 键 词：改良后高脂饮食 非酒精性脂肪性肝病 大鼠 动物模型 

分 类 号：R575[医药卫生—消化系统]