北沙参及易混品的ITS2分子鉴定  

Identification of Glehniae Radix and its adulterants by ITS2 sequence

作　　者：李忠祥[1] 王育莹[1] 原忠[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2013年第10期803-806,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的北沙参(Glehniae Radix)药材性状与桔梗科(Campanulaceae)的南沙参(Adenophora tetraphylla(Thunb.)Fisch.)、轮叶党参(Codonopsis lanceolata(Sieb et Zucc)Benth.et Hook.)等类似,易产生混淆。作者通过对不同产地北沙参及其混淆品的ITS2(Internal Transcribed Spacer2)分子鉴定,探讨ITS2条形码序列对北沙参药材鉴定可行性。方法对样品进行DNA提取,并运用PCR扩增方法进行ITS2序列扩增,采用双向测序的方法对PCR产物进行测序。测序结果运用DNAMAN软件对测序结果进行拼接,用MEGA5.1软件对序列结果进行分析并建立K2P模型的NJ树,并预测ITS2的二级结构。结果与结论不同产地北沙参的ITS2序列一致,并且从NJ树可明显区分北沙参及其混淆品。Objective To report ITS2 barcoding as a powerful identification method for Glehniae Radix and its adulterants. Methods The PCR technique was performed to get the ITS2 regions and purified PCR prod ucts were sequenced. Sequences assembly and consensus sequence generation were performed using the DNAMAN. The genetic distances were computed using MEGA 5.1 in accordance with the K2P model. The phylogenetic tree was constructed using the NJ and K2P methods. The ITS2 secondary structures of the sam ples were also developed. Results and Conclusions ITS2 sequences of Glehnia Radix from different origin were the same. Based on the NJ tree and their ITS2 secondary structures, Glehnia Radix and its adulterants could be easily identified.

关 键 词：北沙参 混淆品 南沙参 轮叶党参 ITS2分子鉴定 

分 类 号：R282.5[医药卫生—中药学]