重组人血管内皮抑素对视网膜新生血管的抑制作用  

Inhibitory effects of recombinant human endostatin on retinal neovascularization

作　　者：李彩云[1] 张奕霞[2] 陈宁宁[1] 李燕利[1] 张晖[1] 

机构地区：[1]石河子大学医学院,2010级新疆石河子832000 [2]石河子大学医学院第一附属医院眼科,新疆石河子832000

出　　处：《中华实用诊断与治疗杂志》2013年第10期990-992,共3页Journal of Chinese Practical Diagnosis and Therapy

摘　　要：目的探讨重组人血管内皮抑素(恩度)对高氧诱导的小鼠视网膜病变模型中新生血管的抑制作用。方法将60只C57BL/6J小鼠随机分为正常对照组、高氧对照组、生理盐水对照组与恩度干预组,每组15只。除正常对照组外,其余各组均建立视网膜病变模型。正常对照组与高氧对照组小鼠不做处理,生理盐水对照组于第12天给予腹腔注射0.1mL生理盐水,恩度干预组于第12天给予腹腔注射恩度0.1mL(17.5μg),连续注射5d,鼠龄17d时,视网膜组织切片行HE染色计数突破内界膜的新生血管内皮细胞核数目;采用免疫组织化学染色法检测视网膜中血管内皮生长因子表达情况。结果高氧对照组和生理盐水对照组突破内界膜的新生血管内皮细胞核数目及血管内皮生长因子表达阳性率明显高于正常对照组及恩度干预组(P<0.01),恩度干预组高于正常对照组(P<0.05)。结论恩度可抑制小鼠模型中视网膜新生血管形成,有可能成为防治血管增生性视网膜病变的一种方法。Objective To explore the inhibitory effects of recombinant human endostatin （Endostar） on retinal neovascularization of hyperoxia-induced retinopathy （OIR） models in mice. Methods Sixty C57BL/6J mice were randomly divided into normal control group, high oxygen control group, saline control group and Endostar intervention group, with 15 mice in each group. All groups were established OIR models except the normal control group. The saline control group was given intraperitoneal injection of 0.1 mL normal saline by day 12, and Endostar intervention group was given intraperitoneal injection of 0. 1 mL （17.5 μg） Endostar by day 12, totally for 5 days. When the mice were 17 days old, the retinal tissue slice was performed HE stain to calculate the number neovessel endotheliocyte nuclei extending from retina to vitreous. The vascular endothelial growth factor （ VEGF ） expression was detected by using immunohistochemistry. Results The number of neovessel endotheliocyte nuclei extending from retina to vitreous and the positive rate of VEGF were significantly higher in high oxygen control group and saline control group than those in normal control group and Endostar intervention group （P〈0.01）, and higher in Endostar group than those in normal control group （P〈0.05）. Conclusion Endostar can inhibit retinal neovascularization in mice models, and may be one of methods to prevent vascular proliferation retinopathy.

关 键 词：视网膜病变 视网膜新生血管 重组人血管内皮抑素 腹腔注射 

分 类 号：R774.1[医药卫生—眼科]