不同转移潜能食管癌细胞株裸鼠模型的建立  

Establishment of Esophageal Cancer Cell Lines with Different Metastatic Potential in Nude Mice Model

作　　者：胡翠梨[1] 张志强[1] 温浩[2] 夏宇[1] 

机构地区：[1]新疆医科大学第一附属医院消化科,830054 [2]新疆医科大学第一附属医院肝胆外科,830054

出　　处：《胃肠病学》2013年第9期548-551,共4页Chinese Journal of Gastroenterology

基　　金：新疆维吾尔自治区自然科学基金(2010211B20)资助

摘　　要：背景:食管癌的转移率较高,是导致患者死亡的主要原因,但其机制仍不完全清楚。目的:建立具有不同转移潜能的高侵袭能力食管癌细胞株亚系裸鼠模型。方法:应用Transwell侵袭小室从食管癌细胞株Eca-109中筛选出高侵袭能力的食管癌细胞株亚系。观察母系和亚系细胞形态,MTT法检测细胞增殖能力的变化,划痕法检测细胞迁移能力。将食管癌Eca-109细胞及其亚系Eca-109 T4细胞分别皮下注射于裸鼠体内诱导移植瘤模型,观察成瘤率、成瘤时间、肿瘤生长情况。结果:成功从食管癌Eca-109细胞株中筛选出高侵袭能力的食管癌细胞株亚系Eca-109 T4,两者细胞形态无明显差异。与Eca-109细胞相比,Eca-109 T4细胞增殖能力和划痕愈合能力均明显增强。Eca-109组和Eca-109 T4组裸鼠成瘤率均为100%,与Eca-109组相比,Eca-109 T4组裸鼠成瘤时间更早且瘤体生长更快。结论:成功建立了不同转移潜能的高侵袭能力食管癌细胞株亚系裸鼠成瘤模型,为食管癌转移的进一步研究提供了理想模型。Background： Esophageal cancer has a high metastasis rate, which is the main cause of death, but its mechanism is not completely clear yet. Aims： To establish an esophageal cancer cell subline with a metastatic potential of high invasion capacity in nude mice. Methods： Transwell invasion chamber was used to subdivide esophageal cancer cell subline from esophageal cancer cell line Eca-109. The cell morphology of parent line and subline was examined. MTT method was used to detect changes of cell proliferation. Cell migration was determined by scratch method. Esophageal cancer Eca-109 cells and its subline Eca-109 T4 cells were subcutaneously injected into nude mice to induce transplantation model, respectively. Tumor formation rate, time for tumor formation, and tumor growth in nude mice were observed. Results： Esophageal cancer cell subline Eca-109 T4 was successfully established. No significant difference in cell morphology was found between Eca-109 cells and Eca-109 T4 cells. Capacities of cell proliferation and migration in Eca-109 T4 cells were significantly higher when compared with Eca-109 cells. Tumor formation rate in nude mice injected with Eca-109 and Eca-109 T4 cells were 100%, time for tumor formation was earlier in Eca-109 T4 nude mice than in Eca-109 nude mice, and tumor growth was significantly increased. Conclusions： An esophageal cancer cell subline with a metastatic potential of high invasion capacity in nude mice was successfully established, which provides an ideal model for the further study of esophageal cancer metastasis.

关 键 词：食管肿瘤 细胞系 肿瘤 小鼠 裸 肿瘤转移 

分 类 号：R735.1[医药卫生—肿瘤]