短波长单色光干预形觉剥夺性近视发展的实验研究  

Intervention of blue light with short wavelength on the progression of form deprived myopia in guinea pigs

作　　者：刘鹏飞[1] 肖林[1] 畅立斌[1] 褚利群[1] 董宁[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院眼科,北京100038

出　　处：《中华实验眼科杂志》2013年第10期925-929,共5页Chinese Journal Of Experimental Ophthalmology

摘　　要：不同波长的光刺激对眼球的生长发育有不同的影响,其中短波长的蓝光发挥抑制作用.研究能否用蓝光刺激来干扰近视的进展对近视的防治研究有重要意义. 目的 观察短波长蓝光照射对豚鼠单眼形觉剥夺性近视（FDM）模型眼各生物学参数变化及巩膜组织改变的作用.方法 将36只2周龄豚鼠饲养在白光环境中,右眼用面罩遮盖法建立FDM模型,然后用随机数字表法将豚鼠分为去剥夺＋蓝光照射组、单纯去剥夺组和持续剥夺组.其中去剥夺＋蓝光照射组16只豚鼠每日定时右眼解除遮盖1h,去剥夺期间,给予波长430 nm蓝光照射；单纯去剥夺组12只豚鼠,每日定时右眼解除遮盖1h；持续剥夺组8只豚鼠饲养在白光环境中.实验后4周用A型超声测量各组豚鼠的前房深度（ACD）、晶状体厚度（LT）及玻璃体腔深度（VCD）,在扩瞳状态下用检影法测量豚鼠眼屈光度.实验结束时各组取2只豚鼠,质量分数10％水合氯醛麻醉后摘除眼球,制作视网膜病理标本.其他豚鼠麻醉后迅速取鼻侧距视神经根部1 mm处巩膜,称量并计算巩膜干质量.结果 实验开始时3个组间豚鼠右眼的屈光度、ACD、LT及VCD的差异均无统计学意义（均P＞0.05）.实验4周后,去剥夺＋蓝光照射组、单纯去剥夺组和持续剥夺组豚鼠右眼的屈光度分别为（＋1.11±0.17）、（＋0.90±0.1 5）和（-2.73±0.19）D,差异有统计学意义（F=1 445.470,P=0.000）；VCD分别为（3.70±0.09）、（3.78±0.11）和（3.91±0.08） mm,差异有统计学意义（F=13.243,P＜0.01）;3个组豚鼠的巩膜干质量分别为（0.61±0.09）、（0.54±0.08）和（0.43±0.07） mg,差异有统计学意义（F=10.458,P＜0.01）；而3个组间ACD、LT的差异均无统计学意义（F=0.203、0.084,P＞0.05）.实验开始时,3个组间豚鼠左眼屈光度、ACD、LT及VCD的差异均无统计学意义（均P＞0.05）,实验4周后,3个组间左眼屈光度的差异有统计Background Light stimulation at different wavelength influences the development of eyes.It has been showed that blue light can inhibit the growth of eyeball.To study whether blue light exposure can delay the extension of myopia is an interested research project.Objective This study was to investigate the effect of blue light with short wavelength on ocular growth in form deprived myopia （FDM） in guinea pigs and provide a new option for the prevention and treatment of myopia.Methods Thirty-six 2-week-old guinea pigs were reared in the environment of white light.The right eyes of the animals were occluded to establish the FDM models.The models were randomized into the deocclusion ＋ blue light exposure group,simple deocclusion group and continuous occlusion group according to the random number table.The right eyes of the models were deoccluded for 1 hour per day to give the blue light （430 nm） irradiation in the deocclusion ＋ blue light exposure group,and the right eyes were deoccluded for 1 hour per day only in the simple deocclusion group.In the continuous occlusion group,the right eyes of the models were occluded until the end of this experiment.Anterior chamber depth （ACD）,lens thickness （LT） and vitreous cavity depth （VCD） were measured by A-type sonography.The binocular diopter of the guinea pigs was detected using retinoscopy in the mydriatic condition.In the fourth week after experiment,the retinal sections were prepared for the regular histopathological examination,and the scleral tissues next to 1 mm from optical nerve were exacted to obtain the dry weight of scleral tissues.Results In the right eyes of the animals,no significant differences were found in the diopter,ACD,LT and VCD before experiment among the 3 groups （all at P〉0.05）.At the end of experiment,the refraction of right eye in the deocclusion ＋ blue light exposure group,simple deocclusion group and continuous occlusion group was （＋1.11±0.17）D,（＋0.90±0.15）D and （-2.73±0.19）D respectively,with a

关 键 词：蓝光 短波长 屈光不正 形觉剥夺性近视 眼球发育 巩膜 豚鼠 

分 类 号：R778[医药卫生—眼科]