雌雄激素对去卵巢雌鼠泪腺中基质金属蛋白酶-2表达的影响  

Effects of estrogen and androgen on expression of metalloproteinase-2 in lachrymal gland in ovariectomizedrats

作　　者：赵萍[1] 宋秀君[2] 赵霞[2] 

机构地区：[1]河北医科大学第四医院眼科,石家庄050011 [2]河北医科大学第三医院眼科,石家庄050051

出　　处：《中华实验眼科杂志》2013年第10期944-948,共5页Chinese Journal Of Experimental Ophthalmology

摘　　要：背景 干眼的发病机制比较复杂,性激素的改变被认为是其影响因素之一,性激素在许多组织中调节基质金属蛋白酶（MMPs）的合成,然而性激素在泪腺中MMP-2的表达作用却不十分清楚. 目的 观察雌激素、雄激素对去卵巢雌鼠泪腺中MMP-2表达的影响,探讨MMP-2在干眼中的作用机制.方法 采用随机数字表法将清洁级雌性性成熟Wistar大鼠64只随机分为正常对照组8只、伪手术组8只及实验组48只.伪手术组大鼠仅打开腹腔切除部分脂肪,实验组大鼠行双侧卵巢切除术（OVX）.OVX术后5个月采用随机数字表法将实验组随机分为模型对照组、药物赋形剂组、全身雌二醇治疗组、雌二醇点眼组、全身睾丸酮治疗组和睾丸酮点眼组,每组8只大鼠,分别全身及局部给予玉米油、苯甲酸雌二醇、丙酸睾丸酮药物.给药6周后摘取大鼠泪腺,应用逆转录-PCR（RT-PCR）法检测各组大鼠泪腺中MMP-2 mRNA的表达,采用Westernblot法检测大鼠泪腺中MMP-2蛋白含量（MMP-2/GAPDH）的变化. 结果 RT-PCR检测表明,全身雌二醇治疗组大鼠泪腺中的MMP-2 mRNA表达量最强,而全身睾丸酮治疗组泪腺中的MMP-2 mRNA表达量最弱.各组间MMP-2 mRNA表达量（MMP-2 mRNA/β-actin mRNA）的差异有统计学意义（F=18.60,P＜0.01）,其中模型对照组大鼠泪腺中MMP-2 mRNA表达水平明显高于正常对照组（0.66±0.10 vs.0.47±0.10）,差异有统计学意义（q=3.01,P＜0.05）；全身雌二醇治疗组大鼠泪腺中MMP-2 mRNA表达值为0.83±0.10,明显高于模型对照组,差异有统计学意义（q=2.79,P＜0.05）,而全身睾丸酮治疗组大鼠泪腺中MMP-2 mRNA表达值为0.12±0.04,明显低于模型对照组,差异有统计学意义（q=11.41,P＜0.0i）.Western blot法检测结果显示,全身雌二醇治疗组大鼠泪腺中MMP-2蛋白表达最强,而全身睾丸酮组泪腺中的MMP-2蛋白表达量最弱.各组间大鼠泪腺中MMP-2蛋白表达差异有统计学意义（F=Background The pathogenesis of dry eye is complicated,hormone level is thought to be one of impact factors in the development of dry eye.The regulation of the synthesis process of metalloproteinases（MMPs） in tissue has been reported.However,the effects of hormone on expression of MMP-2 in lachrymal gland is not clear.Objective This study was to investigate the effects of estrogen and androgen on the expression of MMP-2 in lachrymal gland in ovariectomized rats,and explore the role of MMP-2 in dry eye.Methods Sixty-four 3-monthold clean female Wistar rats were randomized into control group（8 rats）,sham operation group（8 rats）and experiment group（48 rats）.Ovariectomy（OVX） was performed on the rats of experiment group,and only fat tissue of abdominal cavity was cut off in the rats of the sham operation group.After 5 months of OVX,the experimental rats were subdivided into model control group,vehicle group,estrogen and androgen systemic or topical utilization groups and 8 rats for each group.Six weeks after administration of the drugs,the lachrymal gland was obtained.The expression of MMP-2 mRNA in the lachrymal gland was detected by reverse transcription PCR（RT-PCR）,β-actin mRNA was used as an internal control,and the expression of MMP-2 protein was detected by Western blot,GAPDH was used as protein loading control.The use and care of the rats complied with the ARVO Statement.Results The expression of MMP-2 mRNA was strongest in the systemic estrogen group and was weakest in the systemic androgen utilization group.A significant difference in the MMP-2 mRNA expression was found among the 8 groups（F=18.60,P〈0.01）,and the MMP-2 mRNA was significantly higher in the model group than that of the normal control group（0.66±0.10vs.0.47±0.10）（q=3.01,P〈0.05）.In addition,the MMP-2 mRNA was significantly higher in the systemic estrogen group compared with the model group （0.83 ±0.10 vs.0.66-0.10） （q =2.79,P〈0.05） ; while the expression of MMP-2 mRNA was significantly declined

关 键 词：干眼 雌激素 雄激素 基质金属蛋白酶 

分 类 号：R777[医药卫生—眼科]