Wnt/β-catenin与绒毛膜癌耐药性之间关系研究  

Study of the relationship between Wnt/β-catenin and drug resistance of choriocarcinoma

作　　者：蔡净亭 唐迪红 胡慧[2] 刘艳琼 夏蛮 张静莉[2] 刘惠宁[2] 

机构地区：[1]湖南省肿瘤医院,中南大学湘雅医学院附属肿瘤医院,长沙410006 [2]中南大学湘雅医院妇产科

出　　处：《中国医师杂志》2013年第8期1009-1012,共4页Journal of Chinese Physician

基　　金：基金项目：国家自然科学基金资助项目（81101995）;湖南省自然科学基金资助项目（13JJ4120）

摘　　要：目的 研究Wnt/β-catenin信号通路的枢纽分子β-连接蛋白（β-catenin）在绒毛膜癌亲本细胞株（JEG-3）及耐药细胞株（JEG-3/VP16）中的表达情况,探讨β-catenin在绒毛膜癌耐药性中所起的作用.方法 分别应用RT-PCR及western blot方法检测JEG-3和JEG-3/VP16细胞株中β-catenin的mRNA和蛋白质的表达情况,并采用流式细胞技术测试出两种细胞株中β-catenin阳性细胞的比例.结果 RT-PCR和western blot结果显示在JEG-3细胞及JEG-3/VP16细胞中均可检测到β-catenin mRNA和蛋白的表达,但JEG-3/VP16细胞中β-catenin mRNA（1.43±0.24）和蛋白的表达（1.49 ±0.17）水平均明显高于JEG-3细胞（0.65±0.14,0.66±0.16,P＜0.01）,而β-catenin在JEG-3/VP16细胞中的阳性表达率[（40.13±5.17）％]明显高于在JEG-3细胞中的阳性表达率[（13.15±1.48）％,P ＜0.01].结论 β-catenin在耐药性绒毛膜癌细胞株JEG-3/VP16中高表达,提示β-catenin可能参与了绒毛膜癌的耐药机制.Objective To investigate the relationship between the expression of β-catenin and drug-resistance mechanism of choriocarcinoma according to the expression of β-catenin in JEG-3 cells （human choriocarcinoma cell line） and drug resistant JEG-3/VP16 cells.Methods The mRNA and protein expressions of β-catenin were analyzed with reverse transcription-polymerase chain reaction（RT-PCR） and Western blotting.Flow cytometry was used to determine the percentages of β-catenin-positive cells in the two choriocarcinoma cell lines.Results Both drug resistant choriocarcinoma cells and drag sensitive cells were found to express β-catenin; but the expression of β-catenin mRNA （1.43 ±0.24） and protein（1.49 ±0.17）in drug resistant choriocarcinoma cells was found much higher than that in drug sensitive cells（0.65 ±0.14,0.66 ±0.16,P 〈0.01）.And according to detect by flow cytometry,we found the number of β-catenin-positive cells in JEG-3/VP16 cells [（40.13 ±5.17） %] was much more than that in JEG-3 cells [（13.15 ± 1.48） %,P 〈 0.01].Conclusions β-catenin was highly expressed in the drug resistant choriocarcinoma cell line （JEG-3/VP16）.It indicates β-catenin might be involved in the drug resistance mechanism of choriocareinoma.

关 键 词：信号传导 反式激活因子类 细胞支架蛋白质类 绒毛膜癌 药物耐受性 

分 类 号：R737.33[医药卫生—肿瘤]