维生素对大肠杆菌Escherichia coli.JN8产L-色氨酸的影响  

Effect of Vitamins on Production of L-Tryptophan in Escherichia coli.JN8

作　　者：娄秀平[1] 沈健增 蔡宇杰[1] 廖祥儒[1] 张大兵[2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江苏汉邦科技有限公司,江苏淮安223001

出　　处：《食品与生物技术学报》2013年第9期921-926,共6页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(21275066);江苏省科技创新与成果转化项目项目(重大科技支撑与自主创新专项BY2010117)

摘　　要：研究了9种维生素对大肠杆菌Escherichia coli.JN8生长及其发酵产L-色氨酸产量的影响,并对发酵培养基中的维生素浓度进行了优化。结果表明,低浓度肌醇和叶酸可显著促进大肠杆菌的生长及L-色氨酸的合成,叶酸和肌醇的最适添加量分别为0.000 2 mg/L和0.2 mg/L;较高浓度的生物素可以明显促进菌体生长及色氨酸的合成,其最适添加量为0.12 mg/L;较低浓度VB6的添加严重抑制色氨酸的合成,当其质量浓度增至0.8 mg/L时,菌体浓度和色氨酸的产量均较对照有显著的提高,分别提高11.8%和27.2%。添加VB5可略微提高色氨酸的产量,最优质量浓度为0.4 mg/L。添加VB1、VB2、VB3和VC虽然对菌体的生长均有较强的促进作用,但对于色氨酸合成却有不同程度的抑制作用。In this manuscript,the effect of 9 kinds of vitamins on overproduction of L-tryptophan and cell growth were studied and the concentration of vitamins in culture medium was also optimized. The results showed that the cell growth and the synthesis of L-tryptophan were both promoted strongly with the addition of low concentration of inositol and folic acid. Their optimum concentrations were 0.000 2 mg/L, 0.2 mg/L, respectively. Adding higher concentration of biotin can significantly promote cell growth and the synthesis of L-tryptophan ,the optimum dosage was 0.12 mg/L. The synthesis of L-tryptophan was inhibited severely by adding lower concentration of VB6,when the optimal added concentration was 0.8 mg/L,cell concentration and L-tryptophan production have been significantly improved by 11.8% and 27.2% compared with the control, respectively. Adding VB5 may slightly improve the yield of the L-tryptophan,the optimal concentration was 0.4 mg /L. Although ,adding VB1,VB2,VB3 and VC,cell growth was improved strongly, the synthesis of L-tryptophan were inhibited by different degrees.

关 键 词：维生素 大肠杆菌Escherichia coti JN8 L-色氨酸 

分 类 号：TQ922[轻工技术与工程—发酵工程]