NR2A基因启动子区GT二核苷酸短串联重复序列多态性与精神分裂症相关性研究  

Association between the NR2A gene (GT)n Polymorphisms and Heroin Dependence

作　　者：党伟[1] 王珊[1] 刘显阳[1] 贾杰[1] 朱永生[2] 张茜[2] 

机构地区：[1]西安市精神卫生中心,陕西西安760060 [2]宁夏医科大学教育部生育力保持重点实验室,宁夏银川750004

出　　处：《现代生物医学进展》2013年第27期5226-5229,共4页Progress in Modern Biomedicine

基　　金：西安市科技计划项目(SF1210(1-d));国家自然科学基金项目(31100900)

摘　　要：目的:NR2A基因是精神分裂症的重要候选基因,本研究旨在探讨NR2A基因启动子区GT二核苷酸短串联重复序列多态性与精神分裂症相关性。方法:根据DSM-IV诊断标准,随机选取陕西汉族无亲缘个体420例精神分裂症患者(精神分裂症组)及410例体检健康者(正常对照组)提取基因组DNA,采用特异荧光物质羧基荧光素FAM标记引物,聚合酶链反应(PCR)扩增,377测序仪基因扫描电泳分型,采用SPSS 16.0统计软件分析各等位基因在组间的差异。结果:发现NR2A基因GT基因座在830个无关个体中共检测出19种等位基因,精神分裂症组(GT)21、(GT)22、(GT)23等位基因频率显著低于正常对照组(P<0.05);精神分裂症组(GT)26等位基因频率显著高于正常对照组(P<0.05)。结论:NR2A基因启动子区GT二核苷酸短串联重复序列多态性可能通过影响NR2A蛋白的表达从而对精神分裂症的发生产生发展影响,(GT)21、(GT)22、(GT)23可能是精神分裂症的保护等位基因,而携带有(GT)26等位基因的个体个能更容易患精神分裂症。Objective： To study a possible association between NR2A gene GT Polymorphisms and heroin dependence. Methods： The diagnosis of schizophrenia was based on the Diagnostic and Statistical Manual of Mental Disorders （DSM）-IV criteria. Genomic DNA was isolated from the venous blood leukocytes of 420 unrelated patients with schizophrenia （Case group） and 410 healthy unrelated individuals （Control group）. The primers specific for the （GT）n repeat was used to amplify the repeat-containing genomic fragment which were a 6-carboxyfluorescein （FAM）-labeled upstream primer. The （GT）n were genotyped by ABI DNA 377 sequencer. Allele frequen- cies were analyzed by SPSS 16.0 sottware. Results： Nineteen alleles are found in （GT）n locus in 830 Chinese Han population of Shannxi. The allele （GT）21, （GT）22, （GT）23 of （GT）n were significantly lowerer than controls （P〈0.05）. The allele （GT）26 of （GT）n was significantly higher than controls. Conclusion： These findings point to a role for NR2A （GT）n polymorphisms in schizophrenia among the Han Chinese in Shannxi, The （GT）21, （GT）22, （GT）23 of （GT）n may be protective factor. However, the individuals with （GT）26 are susceptible to heroin dependence.

关 键 词：NR2A基因 单核苷酸多态性 精神分裂症 

分 类 号：R749.3[医药卫生—神经病学与精神病学] R394.3[医药卫生—临床医学]