血浆microRNA在早期乳腺癌诊断中的价值(英文)  

The Diagnosis value of Plasma microRNAs for Primary Breast Cancer

作　　者：李振凤[1] 葛银林[1] 张金玉[1] 赵辉 曹晓枫 郑征[1] 

机构地区：[1]青岛大学医学院生物化学与分子生物学教研室,山东青岛266021 [2]青岛市中心医院乳腺科,山东青岛266021

出　　处：《现代生物医学进展》2013年第27期5310-5314,共5页Progress in Modern Biomedicine

基　　金：Qingdao Science and Technology Project(08-2-1-4-nsh)~~

摘　　要：目的:探讨不同microRNA的表达及评估血浆microRNA作为早期乳腺癌诊断的新标志物的价值。方法:我们收集了49例早期乳腺癌的术前血浆作为实验样本和36例健康女性血浆作为对照样本。应用反转录和实时荧光定量聚合酶链反应,我们检测miR-21,miR-205,miR-222这三个候选基因的相对表达量并分析了这三个候选microRNA表达与临床病理特征的关系。结果:我们发现,与健康对照相比,miR-21(1.565,P=0.022)and miR-222(2.258,P<0.001)在乳腺癌病人血浆中的表达明显升高,而miR-205(0.591,P=0.001)在乳腺癌病人血浆中的表达明显下降。并且在临床病例资料数据的比较中,miR-21(P=0.0101)在乳腺癌病人中的表达水平与雌激素受体和孕激素受体相关。血浆中miR-222的表达水平在肿瘤不同分期中明显不同。结论:本实验证明miR-21,miR-205 and miR-222的表达水平与乳腺癌的病理特征明显相关,可以作为乳腺癌的潜在标志物。Objective： To investigate the expression of differential microRNAs and estimate the value of circulating microRNAs as novel biomarkers for primary breast cancer detection. Methods： The preoperative plasma samples of 49 primary breast cancer patients were chosen as experiment group while a set of 36 healthy women plasma as control samples. The relative expression of three candidate miRNAs （miR-21, miR-205, miR-222） was detected using Reverse Transcription and quantitative Real-time Polymerase Cycle Reaction （RT-qPCR）, and the relationship between microRNA expressions and clinical histopathological features was analyzed. Results： It was found that the expresion levels ofmiR-21 （1.565, P=0.022） and miR-222 （2.258, P〈0.001） were significantly higher in plasma of breast cancer patients compared with those of healthy controls, while the level of miR-205 （0.591, P=0.001） was significantly lower in patients. Moreover, the comparison with the clinicopathologic data of the BC patients showed that increased levels ofmiR-21 （P=0.010t） was as- sociated with estrogen receptors and progesterone receptors. The plasma levels of miR-222 （P〈0.0001） was significantly different with stages. Conclusions： The expression ofmiR-21, miR-205 and miR-222 had close correlation with the clinicopathologic features of breast cancer, which indicated that miRNA can be used as a potential marker for breast cancer.

关 键 词：MICRORNA 乳腺癌 血浆 诊断 

分 类 号：R737.9[医药卫生—肿瘤]