锌对缺血再灌注大鼠肝脏金属硫蛋白-1基因表达的影响  

EFFECT OF ZINC ON THE GENE EXPRESSION OF HEPATIC METALLOTHIONEIN-1 AFTER ISCHEMIA REPERFU-SION INJURY IN RATS

作　　者：蒋与刚[1] 郭长江[1] 葛玉章[1] 韦京豫[1] 王冬兰[1] 高兰兴[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《营养学报》2000年第4期294-297,共4页Acta Nutrimenta Sinica

摘　　要：目的　研究锌对肝脏缺血再灌注损伤 (HIRI)保护作用的分子机制。方法　采用RT- PCR技术 ,检测分析金属硫蛋白 - 1 (MT- 1 )基因在 HIRI大鼠肝组织中的表达和锌对其表达的调节。结果　各组大鼠肝组织均有MT- 1基因表达 ;缺血 30 min、再灌 90 min大鼠肝组织中 MT- 1m RNA较对照组显著降低 (P<0 .0 1 ) ;灌胃补锌后 ,HIRI大鼠肝 MT- 1 m RNA转录水平较对照组明显增高 (P<0 .0 1 ) ,单纯补锌亦可上调其组织中 MT- 1基因表达 (P<0 .0 1 )。结论　在锌对HIRI产生保护作用的分子机制中 ,调节其肝组织中 MT- 1转录水平的表达可能是一条重要途径。Objective:[WT5BZ]To explore the molecular mechanism of protection of zinc against hepatic ischemia reperfusion injury(HIRI). [WT5HZ]Methods:[WT5BZ]The expression of hepatic metallothionein 1(MT 1)gene and regulation by zinc were determined by RT PCR(reverse transcription polymerase chain reaction)in HIRI rats. [WT5HZ]Results:[WT5BZ]1.Hepatic MT 1 mRNA was expressed in all groups;2.The level of hepatic MT 1 mRNA in HIRI group(ischemia 30 min,reperfusion 90 min)was significantly lower than control.After zinc supplementation,the content of hepatic MT 1 mRNA was increased significantly;3.The hepatic MT 1 expression was also enhanced by zinc in normal rats. [WT5HZ]Conclusion:[WT5BZ]The results of our studies suggest that the regulation of hepatic MT 1 genes by zinc is one of the main ways contributed to the mechanism of protection by zinc in HIRI.

关 键 词：金属硫蛋白-1 反转录聚合酶链式反应 肝脏缺血再灌注 锌 

分 类 号：R363[医药卫生—病理学]