生物转化合成左旋紫堇达明诱导剂筛选及优化  

Screening and Optimality of Inducer Biologically Converting to Synthesize l-CDL

作　　者：宋洋[1] 王严[1] 余伯阳[1] 刘吉华[1] 

机构地区：[1]中国药科大学中药复方研究室,江苏南京211198

出　　处：《中国野生植物资源》2013年第5期7-9,20,共4页Chinese Wild Plant Resources

基　　金：重大新药创制专项--新型强效快速镇痛药物紫堇达明临床前研究(2012ZX09J12110-06B)资助

摘　　要：目的:左旋紫堇达明为中药延胡索中痕量高效镇痛活性成分,灰色链霉菌(Streptomyces griseus ATCC 13273)具有生物转化左旋四氢巴马汀产生左旋紫堇达明的转化活性。筛选合适的生物转化诱导剂并优化诱导条件,提高左旋紫堇达明的转化产率。方法:以左旋紫堇达明的转化产率为指标,选用苯甲酸、正己烷、苯巴比妥钠作为诱导物,筛选合适的诱导物并优化转化体系诱导条件。结果:苯甲酸、正己烷、苯巴比妥钠均能显著提高左旋紫堇达明的转化产率。结论:正己烷廉价易得且不影响转化产物分离,适合工业化生产使用,为该转化体系的合适诱导剂。Objective： /-corydalmine （/-CDL）, is proved to show high analgesic activity. However, 1- CDL falls into the category of trace ingredients of traditional Chinese medicine Corydalis Rhizoma. Previ- ous research indicated that Streptomyces griseus ATCC 13273 catalyzed the microbial transformation process of/-THP to produce /-CDL. The suitable inducers of the transformation systerm were screened and the induction conditions were optimized to improve the transformation rate of/-CDL. Methods： Ben- zoic acid, n-Hexane and phenobarbitol sodium were investigated as inducers. HPLC-UV method was em- ployed to detect the transformation rate of/-CDL. Finally, the suitable inducing time and concentration were ensured. Results： Benzoic acid, n-Hexane and phenobarbitol sodium all improved the transforma- tion rate markedly. Conclusion： N-Hexane was a suitable inducer for this transformation systerm because of price and acquisition, easy product isolation, especially perfect for industrial-scale production.

关 键 词：左旋紫堇达明 灰色链霉菌ATCC 13273 诱导剂 左旋四氢巴马汀 

分 类 号：TQ92[轻工技术与工程—发酵工程]