卵巢去势对大鼠牙髓组织成牙/成骨能力的影响  

Effects of ovariectomy on the odonto/osteogenic capacity of rat incisor pulps

作　　者：王燕萍[1,2] 宋卫健[1,2] 曹灵[3] 于金华[4] 

机构地区：[1]苏州大学附属口腔医院 [2]苏州口腔医院口腔内科,江苏苏州215005 [3]南京医科大学附属口腔医院儿童口腔预防科,江苏南京210029 [4]南京医科大学口腔医学研究所.附属口腔医院牙体牙髓病科,江苏南京210029

出　　处：《口腔生物医学》2013年第3期117-120,共4页Oral Biomedicine

基　　金：江苏省卫生厅医学重点人才项目(RC2011140);江苏高校优势学科建设工程资助项目(2011-137)

摘　　要：目的:观察卵巢去势1个月对大鼠切牙牙髓组织中成牙、成骨指标的影响。方法:将10只成年雌性SD大鼠(8周龄)随机分为假手术组及卵巢去势组,每组各5只;在全麻下,将去势组大鼠双侧卵巢摘除,假手术组行相同切口,保留卵巢;1个月后,处死大鼠,分离牙髓组织,通过实时定量RT-PCR及Western blot方法检测两组大鼠牙髓组织中成牙、成骨相关指标的表达。结果:去势组牙髓组织中,Runt相关转录因子2(Runt-related transcription factor 2,RUNX2)、骨细胞特异性转录因子(Osterix,OSX)、骨钙素(Osteocalcin,OCN)、牙本质涎蛋白(dentin sialoprotein,DSP)、牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)的基因和/或蛋白表达等成牙及成骨指标均较假手术组牙髓低,而碱性磷酸酶(alkaline phosphatase,ALP)的表达无明显改变。结论:卵巢去势1个月可以明显降低大鼠切牙牙髓组织中成牙、成骨指标的表达,提示卵巢去势下调了牙髓细胞的成牙及成骨能力。Objective：To investigate the effects of ovariectomy on the expression of odonto/osteogenic markers in rat incisor pulps. Methods ： Ten 8-week-old female SD rats were randomly divided into 2 groups ： Ovariectomized（OVX） group （ n = 5 ） and Sham-opera- tion（Sham） group （tt = 5）. All rats were anesthetized and both ovaries of OVX-rats were gently removed. Sham-operation rats were treated with the same incisions to expose the ovaries but without any hurt to them. One month after the operation, the incisor pulps were respectively isolated. Real-time RT-PCR and Western blot were performed to evaluate the expression of odonto/osteogenie markers in the two groups. Results：The expression of odonto/osteogenic markers （i. e. , RUNX2/RUNX2, OSX/OSX, OCN/OCN, and DSPP/ DSP） in OVX group was significantly weaker than that in Sham group. However,the expression of ALP was not significantly changed. Conclusions：Ovarieetomy can downregulate the expression of odonto/osteogenic markers in dental pulps, indicating that ovarieetomy decreased the odonto/osteogenie capacity of dental pulp ceils.

关 键 词：卵巢去势 牙髓 成牙 成骨 

分 类 号：R780.2[医药卫生—口腔医学]