细胞凋亡及p53基因的表达在C57BL/6N系小鼠腭裂形成中的意义  

The significance of PCD and mRNA transcription of p 53 gene during development of normal palate and cleft palate in C57BL/6N mouse

作　　者：李鑫[1] 金岩[1] 董绍忠[1] 朱萧玲[1] 

机构地区：[1]第四军医大学口腔医学院病理科,710032

出　　处：《实用口腔医学杂志》2000年第6期440-442,共3页Journal of Practical Stomatology

摘　　要：目的 :探讨细胞程序性死亡和相关基因 p5 3的表达变化在腭裂发生中的意义。方法 :利用建立的腭裂模型 ,采用原位末端转移酶标记法 (TUNEL)和基因探针原位杂交法 ,原位观察了二种阳性信号的表达。结果 :实验组小鼠腭突发育的垂直期、上抬期中细胞程序性死亡明显多于对照组 (P<0 .0 1)。但两组间 p5 3m RNA转录水平的变化均无明显差异 (P>0 .0 5 )。对照组小鼠生长早期 p5 3基因的表达高于同组融合期的腭突。结论 :在腭突发育时期出现超出生理范围的细胞程序死亡 ,影响腭突以后形态、体积的发育 ,与腭裂形成有密切关系。p5 3基因在两组中表达无明显变化 ,提示腭突间充质细胞的程序性死亡 ,可能是通过非 p5砄bjectives:To observe the difference of programmed cell death (PCD) and the expression of related gene p 53 in the development of normal palate and in the formation of cleft palate.Methods:The model of the development of cleft palat was estabished with retinoid acid (80 mg·kg 1 for each pregnant mouse). The palate samples were obtained at GD13 14 (at 13rd day 14 hours of prenancy),13 22 ,14 8,14 14 ,14 33 ,15 8,15 22 and 16 8 respectively.PCD was detected with TUNEL staining,while the mRNA transcription of p 53 was observed by in situ hybridization.Rssults:In early development of palate process,the positive index of PCD in the cleft palate samples was significantly higher than that in the normal ( P <0.01).But no difference was observed in the expression of p 53 ( P >0.05) between the two groups.Conclusion:The proliferation of mesenchymal cells of the early developmental palate process may be inhibited due to the abnomal PCD in the formation of cleft palate.The mechanism of PCD in this study may be a p 53 independed pathway.

关 键 词：细胞程序性死亡 P53基因 腭发育 腭裂 

分 类 号：R780.2[医药卫生—口腔医学]