TLR9激动剂对胰腺癌细胞增殖的影响  

Effect of TLR9 agonist on proliferation of pancreatic cancer cells

作　　者：侯雯跻[1] 范昕[1] 党胜春[1] 祝文蕊 沈耀[1] 蒋海华[1] 刘宇[1] 张建新[1] 

机构地区：[1]江苏大学附属医院普通外科,212001

出　　处：《江苏医药》2013年第19期2233-2235,F0003,I0001,共5页Jiangsu Medical Journal

基　　金：国家自然科学基金(81070287);镇江市科技计划项目(SH2011026;SH2012031)

摘　　要：目的观察Toll样受体9(TLR9)激动剂CPG-寡核苷酸1826(CPG-ODN1826)对体外胰腺癌细胞株PANC-1增殖的影响。方法实验组用CPG-ODN1826干预PANC-1细胞,对照组单独培养PANC-1细胞。采用MTT法、结晶紫实验和软琼脂糖细胞克隆形成实验分析PANC-1细胞增殖能力,Western blot检测磷酸化细胞外调节蛋白激酶(p-ERK)和磷酸化c-Jun氨基末端激酶(p-JNK)的表达。结果与对照组相比,实验组PANC-1细胞增殖能力提高(P<0.05),细胞克隆数增多[(35.60±2.70)个vs.(50.40±4.04)个](P<0.05),p-ERK和p-JNK的表达增强(0.455±0.057vs.0.665±0.121和0.301±0.028vs.0.656±0.051)(P<0.05)。结论 TLR9激动剂CPGODN1826能够促进胰腺癌细胞增殖。Objective To detect the effect of Toll-like receptor 9 （TLR9） agonist CPG- oligonucleotide 1826（CPG-ODN1826） on the proliferation of pancreatic cancer PANC-1 cells in vitro. Methods The PANC-1 cells were divided into group A（intervened with CPG-ODN1826） and B （treated with culture medium as the control）. The proliferation of PANC-1 cells was analyzed by MTT assay, crystal violet test and soft agar colony formation test, respectively. The expressions of phosphorylated extracellular signal-regulated kinase （p-ERK） and phosphorylated c-Jun N-terminal kinase（p-JNK） were detected by Western blot. Results Compared with group B, the proliferation of PANC-1 cells was promoted（P〈0. 05）, the number of cell clone was increased （35.60±2.70 vs. 50.40±4.04） （P〈0. 05） and the expressions of p-ERK and p-JNK were enhanced（0. 455±0. 057 vs. 0.665±0.121 and 0.301±0.028 vs. 0.656±0.051） in group A（P〈0.05）. Conclusion TLR9 agonist CPG-ODN1826 can promote the proliferation of pancreatic cancer cells.

关 键 词：TOLL样受体9 胰腺癌细胞 CPG-寡核苷酸1826 

分 类 号：R735[医药卫生—肿瘤]