不同中医证型原发性肝癌患者介入治疗前后血清蛋白指纹图谱的观察  

Observation of Serum Protein Fingerprinting in Primary Liver Cancer Patients of Different Chinese Medical Syndromes before and after Interventional Treatment

作　　者：杨素芳[1] 邱颂平[1] 刘清华[1] 

机构地区：[1]福建中医药大学药学院,福州350108

出　　处：《中国中西医结合杂志》2013年第10期1352-1355,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：福建省科技厅资助项目(No.2011J01204)

摘　　要：目的应用表面加强激光解析电离化飞行时间质谱(surface enhanced laser desorption ionization time of flight mass spectrometry,SELDI-TOF-MS)蛋白质芯片检测不同中医证型原发性肝癌患者介入治疗前后血清蛋白指纹图谱的变化。方法将154例原发性肝癌患者分为肝郁证、脾虚证、湿热证、血瘀证及阴虚证5组,应用SELDI-TOF-MS分析其血清蛋白指纹图谱,观察肝癌介入治疗前后不同中医证型与血清蛋白指纹图谱之间的关系。结果与健康对照组比较,符合叶氏诊断模型者,肝郁证中质荷比(M/Z)为4 182 Da及6 589 Da、湿热证中M/Z为5 710 Da、阴虚证中M/Z为6 992 Da的差异蛋白表达下调;脾虚证中M/Z为5816 Da、血瘀证中M/Z为4 297 Da的差异蛋白表达上调;差异均有统计学意义(P<0.01);与介入前比较,符合叶氏诊断模型者,肝郁证中M/Z为4 182 Da及6 589 Da、湿热证中M/Z为5 710 Da、脾虚证中M/Z为5816 Da、血瘀证中M/Z为4 297 Da的差异蛋白表达下调;阴虚证中M/Z为6 992 Da的差异蛋白表达上调;差异亦有统计学意义(P<0.05,P<0.01)。结论不同中医证型原发性肝癌患者介入治疗前后的血清蛋白指纹图谱变化有统计学意义。Objective To explore changes of serum protein fingerprinting in primary liver cancer （PLC） patients of different Chinese medical syndromes before and after interventional treatment detected by surface enhanced laser desorption ionization time of flight mass spectrometry （SELDI-TOF-MS）. Methods Totally 154 PLC patients were assigned to 5 groups, i.e., Gan depression syndrome （GDS, 37 cases）, Pi deficiency syndrome （PDS, 45 cases）, dampness heat syndrome （DHS, 18 cases）, blood stasis syndrome （ BSS, 28 cases）, yin deficiency syndrome （YDS, 26 cases）. The mass spectra of serum proteins was analyzed by using SELDI-TOF-MS. Then the correlation between Chinese medical syn- drome types and the mass spectra of serum proteins was explored before and after interventional treat- ment. Results Compared with the healthy control group, the expression of serum proteins peak was down-regulated in GDS with M/Z being 6 589 and 4 182 Da, in DHS with M/Z being 5 710 Da, in YDS with M/ Z being 6 992 Da, while it was up-regulated in PDS with M/Z being 5 816 Da and in BSS with M/Z being 4 297 Da, showing statistical difference （P 〈0.01 ）. Compared with before intervention, the expression of serum proteins peak was down-regulated in GDS with M/Z being 6 589 and 4 182 Da, in PDS with M/Z be- ing 5 816 Da, in DHS with M/Z being 5 710 Da in BSS with MIZ being 4 297 Da, while it was up-regulated in YDS with M/Z being 6 992 Da, showing statistical difference （P 〈0.05, P 〈0.01 ）. Conclusion There was statistical difference in changes of serum protein fingerprinting in PLC patients of different Chinese medi- cal syndromes before and after interventional treatment.

关 键 词：原发性肝癌 蛋白组学 蛋白指纹图谱 

分 类 号：R735.7[医药卫生—肿瘤] R273[医药卫生—临床医学]