益气消癥法对雌激素诱导子宫肌瘤模型豚鼠子宫组织MEK2、p-ERK蛋白表达的影响  

Effect of Reinforcing Qi for Resolving Masses Method on Expressions of MEK2 and p-ERK Protein in Estrogen Induced Uterine Leiomyoma Model Guinea Pigs' Uterine Tissue

作　　者：王娜 宋卓敏[2] 阮氏水[3] 

机构地区：[1]天津市武清区中医院妇科,天津301700 [2]天津中医药大学中医药研究院妇科教研室,天津300193 [3]天津中医药大学,天津300193

出　　处：《中国中西医结合杂志》2013年第10期1408-1411,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30973768)

摘　　要：目的研究益气消癥法对雌激素诱导子宫肌瘤模型豚鼠子宫组织细胞外信号调节激酶2(extracellular signal regulated kinase 2,MEK2)、磷酸化激活丝裂原激活蛋白激酶的激酶(phosphorylation extracellular signal regulated kinase,p-ERK)蛋白表达的影响。方法将去势豚鼠随机分为正常组、模型组、中药高剂量组、中药中剂量组、中药低剂量组及西药组,并以正常组作对照。通过去势加皮下注射雌二醇(estradiol,E2)建立子宫肌瘤模型,通过免疫组化法检测6组子宫组织MEK2、p-ERK蛋白表达。结果模型组豚鼠子宫肌组织MEK2、p-ERK蛋白表达显著高于正常组(P<0.01),中药高、中、低剂量组均有不同程度的降低,其中中药高剂量组豚鼠子宫组织MEK2、p-ERK蛋白表达显著低于模型组及西药组(P<0.01)。结论益气消癥法可能是通过干预MAPK/ERK细胞反应信号通路抑制肌瘤细胞增殖,从而达到治疗子宫肌瘤的目的。Objective To explore the effect of reinforcing qi for resolving masses method （RQRMM） on expressions of extracellular signal regulated kinase 2 （MEK2） and phosphorylation extra- cellular signal regulated kinase （p-ERK） protein in estrogen induced uterine leiomyoma model Guinea pigs＇ uterine tissue. Methods Guinea pigs were randomly divided into five groups, i.e., the model group, the high dose group, the middle dose group, the low dose group, and the Western medicine group （mife- pristone）. The normal control group was set up. The uterine leiomyoma model in guinea pigs was estab- lished by castrating and subcutaneous injecting estradiol （E2）. The protein expression levels of MEK2 and p-ERK of guinea pigs＇ uterine tissues were detected by immunohistochemical assay. Results The protein expressions of MEK2 and p-ERK in the uterine muscular tissue of Guinea pigs＇ uterine tissue were higher in the model group than in the normal group （P 〈0.01 ）. They decreased to some degree in the high dose group, the middle dose group, and the low dose group. Of them, the protein expressions of MEK2 and p-ERK were significantly lower in the high dose group than in the model group and the Western medicine group （P 〈0.01 ）. Conclusion RQRMM could treat uterine leiomyoma possibly through interv ning the MAPK/ERK cell signal pathway to inhibit the proliferation of myoma cells.

关 键 词：益气消癥法 子宫平滑肌瘤 豚鼠 细胞外信号调节激酶2 磷酸化激活丝裂原激活蛋白激酶的激酶 

分 类 号：R737.33[医药卫生—肿瘤] R273[医药卫生—临床医学]