美洲黑杨遗传差异的SRAP和EST-SSR分析  

Genetic Difference Analysis of Populus deltoides Using SRAP and EST-SSR Markers

作　　者：郭娟[1] 樊军锋[1] 梁军[2] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]中国林业科学研究院森林生态环境与保护研究所,北京100091

出　　处：《西北植物学报》2013年第9期1762-1767,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家"十二五"科技支撑专题(2012BAD01B03)

摘　　要：采用SRAP和EST-SSR分子标记对美洲黑杨I-69及与其有亲缘关系的4个美洲黑杨品种进行遗传差异分析,比较两种分子标记在遗传差异性分析中的适用性,为美洲黑杨的鉴别提供准确的分子技术依据。结果表明：（1）以SRAP标记筛选出21对引物组合,共扩增出287条谱带,多态性条带209条,多态性比率72.8%,遗传相似系数为0.548 1~0.769 2。（2）以EST-SSR标记筛选出17对引物,共扩增出86条谱带,多态性条带69条,多态性比率80.2%,遗传相似系数为0.444 4~0.717 2。（3）对SRAP和EST-SSR以及两者混合数据形成的3个遗传相似矩阵进行相关性分析结果显示,SRAP和EST-SSR分别同综合数据之间呈显著相关（r=0.844 2,r=0.830 8）。（4）聚类分析发现,两种分子标记的聚类结果有一定差异,SRAP聚类结果同综合数据分析的结果一致,说明SRAP标记更适用于杨树亲缘关系较近材料的遗传差异分析。Both SRAP and EST-SSR markers were used to analysis genetic difference of 5 Populus deltoides varieties including P.deltoides.cv.‘Lux＇（I-69/55）and 4 varieties related to it.（1）In SRAP analysis,21 primer pairs were screened and 287 bands were amplified,of which 209 bands were polymorphic.The percentage of polymorphic bands was 72.8% and the genetic similarity coefficients ranged from 0.5481 to 0.7692.（2）In EST-SSR analysis,17 primers were screened and 86 bands were amplified,among which 69 bands were polymorphic.The percentage of polymorphic bands was 80.2%and the genetic similarity coefficients ranged from 0.444 4 to 0.717 2.（3）The correlation of the genetic similarity matrix among the SRAP,EST-SSR and the combination data were different,of which the correlation between SRAP and the combination data（r=0.844 2）,EST-SSR and the combination data（r=0.830 8）were significantly correlated.（4）Cluster analysis showed that there was difference between the genetic similarity assessed by SRAP and EST-SSR markers,but the results of SRAP and the combination data were consistent,which showed that SRAP markers were more effective in genetic difference analysis of close Populus lines.

关 键 词：美洲黑杨 SRAP EST-SSR 遗传差异 

分 类 号：Q789[生物学—分子生物学]