生长抑素受体2在结直肠癌中的表达  

Expression of somatostatin receptor type 2 in colorectal cancer

作　　者：程元光[1,2] 吴佩[3] 

机构地区：[1]安徽医科大学第三附院胃肠外科 [2]合肥市第一人民医院 [3]皖南医学院附属弋矶山医院普外科

出　　处：《中国临床药理学与治疗学》2013年第10期1100-1105,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:检测生长抑素Ⅱ型受体(SSR2)mRNA在结直肠癌组织中的表达并研究其在结直肠癌发生、发展中的作用。方法:采用逆转录聚合酶链反应(Reverse transcription-PCR,RT-PCR)方法检测37例结直肠癌患者癌远旁黏膜、癌近旁黏膜、肿瘤组织中SSR2mRNA的表达情况,分析不同组织表达阳性率及表达强度的差别。结果:SSR2mRNA在癌远旁黏膜、癌近旁黏膜、癌组织表达阳性率分别为100%、91.9%、83.8%,表达阳性率差异有统计学意义(P=0.038),结直肠癌组织中SSR2mRNA表达强度明显低于正常黏膜(P=0.021)。在结直肠癌组织中SSR2mRNA的表达阳性率与肿瘤的分化程度、Dukes分期相关(P=0.021,P=0.005),其表达强度也与肿瘤的分化程度、Dukes分期相关(P=0.013,P=0.019),而其表达率及表达强度与病人年龄、性别、肿瘤大小、部位及血清CEA的水平无显著相关性。结论:SSR2mRNA在结直肠癌中表达明显降低,其表达率及表达强度与肿瘤的分化程度、Dukes分期相关,可能与肿瘤的发生、发展有一定的关联。AIM： To investigate the expression and role of somatostatin receptor subtype2 （SSR2） in the pathogenesis and progression of human colorectal cancer （CRC）. METHODS： 37 cases of CRC in inpatients were identified and all tissue specimens of the cases after surgical resec tion immediately were selected. The tissue speci mens including remote mucosa of tumors （10 cm beyond the tumor）, adjacent mucosa of tumors（within 2 cm of the tumor） ,tumors. The expres sion of SSR2 mRNA in the specimens were de tected by reverse transcription polymerase chain reaction（RT-PCR） and the positive rate and the expression in the specimens were analyzed. RE- SULTS： The SSR2 mRNA expression rate of the samples shows remote mucosa of tumor 100%（37/37）,adjacent mucosa of tumor 91.9% （34/ 37） and tumor 83.8% （31/37）. Compared withthe nomal colorectal mucosae, the expression rate and expression intensity of tumor were de- creased significantly （P -- 0. 038, P = 0. 021）. The significant correlation of SSR2 mRNA ex- pression rate and intensity in tumor differentia-tion （P=0. 021,P=0. 013） and tumor stage （P =0. 005,P=0. 019） were found,while there was no significant correlation in the expression of SSR2 mRNA and other clinic pathological pa rameters such as age, sex, tumor size, site and the level of CEA in serum. CONCLUSION： Theexpression of SSR2 mRNA in tumor is decreased significantly. SSR2 mRNA expression rate and expression intensity of tumor are correlated with the tumor differentiation and stage, and SSR2 may play an important role in the pathogenesis and progression of CRC.

关 键 词：大肠癌 生长抑素受体 生长抑素受体2 逆转录聚合酶反应 

分 类 号：R735.34[医药卫生—肿瘤]