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作 者:张娜[1] 柯开富[2] 刘展[1] 邱一华[1] 彭聿平[1]
机构地区:[1]南通大学医学院生理学系,南通226001 [2]南通大学附属医院神经内科,南通226001
出 处:《中华神经医学杂志》2013年第10期994-999,共6页Chinese Journal of Neuromedicine
基 金:国家自然科学基金(81271323);江苏省自然科学基金(BK2011386);江苏高校优势学科建设工程资助项目(PAPD)
摘 要:目的探讨T细胞亚群Thl7细胞是否参与帕金森病fPD)的神经炎症过程。方法C57BL/6小鼠腹腔注射1.甲基L-4-苯基L-1,2,3,6。四氢吡啶(MPTP)制备PD动物模型,用免疫荧光双染分别标记葡萄糖转运体1和CD4^+T细胞、Thl7细胞的核转录因子维甲酸相关孤儿核受体Ⅵ(ROR γt)、白介素(IL)-17,实时定量PCR法检测黑质致密部中IL-17及IL-22mRNA的表达。结果腹腔注射MPTP成功制备PD模型小鼠。在PD模型小鼠中脑黑质致密部中,血管周围见到较多CD4^+T细胞,而且在PD发病后期(MPTP注射后第7天)CD4^+T细胞的渗入比发病早期(MPTP注射后第2天)多,同时ROR矾和IL-17的表达亦增加,两者具有共定位;PD模型小鼠黑质中IL-17及IL-22mRNA表达上调,这种上调在PD发病后期(MPTP注射后第7天)更明显。结论Thl7细胞可侵入PD脑内诱导炎症反应,参与PD的发病机制。Objective To explore whether Thl7 cells play a role in the neuroinflammatory process of Parkinson's disease (PD). Methods C57BL/6 mice were injected intraperitoneally with 1-methyl-4-phenyl-l,2,3,6 tetrahydropyridine (MPTP) to induce PD models. Saline-injected and naive mice were used as controls. In substantia nigra compact (SNc) part, double-irnmunofluorescence staining was used to observe glucose transporter (Glut 1+) and CD4+ T cells or retinoic acid -related orphan nuclear receptor γt (ROR γt) and interleukin (IL)-17. Real-time PCR was employed to examine the mRNA expressions of IL-17 and IL-22 in the SNc. Results PD models were successfully induced in the mice; CD4+ T cells invaded into the brain parenchyma of PD mice models. Besides, the motmt of CD4+ T cells on the seventh day of MPTP injection was more than that of the second day of MPTP injection. The expressions ofROR γt and IL-17 increased in the SNc of the PD mice; a co-localization ofROR γt and IL- 17 in SNc indicated that invasive CD4+ T cells contained Thl 7 cells. There was evident up-regulation in the expressions oflL-17 and IL-22 mRNAs in PD mice as compared with the saline-injected or intact mice, which was much more evident in the late phase of disease (the seventh day of MPTP) than in the early stage (the second day of MPTP injection). Conclusion Neuroinflammation induced by infiltration of Thl 7 cells participates in the process of PD.
关 键 词:帕金森病 TH17细胞 神经炎症 IL-17 IL-22
分 类 号:R742.5[医药卫生—神经病学与精神病学]
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