抗组织胺治疗对实验性肝炎大鼠免疫功能的影响  

Effects of antihistamine treatment on immune fuction in rats with experimental hepatitis

作　　者：李红[1] 赵龙凤[1] 郝彦琴[1] 韩德五[2] 

机构地区：[1]山西医科大学第一医院感染病科,太原030001 [2]山西医科大学肝病研究所

出　　处：《中华肝脏病杂志》2013年第10期764-768,共5页Chinese Journal of Hepatology

基　　金：山西医科大学细胞生理学省部共建教育部重点实验室主任基金（2010-03）;山西省高校科技开发项目（20091174）

摘　　要：目的 研究抗组织胺治疗对实验性肝炎大鼠免疫功能的影响. 方法 取Wistar大鼠30只,随机分为3组：正常对照组（NC组）、实验性肝炎组（EH组）、抗组织胺治疗组（AH组）.EH组采用复合因素造模（用40％四氯化碳油溶液皮下注射,同时辅以低蛋白、低胆碱、高脂肪、高醇饮食）,AH组在EH的基础上给予抗组织胺治疗（酮替芬＋维生素C）.4周末处死动物,取血分别检测血清ALT、总胆红素（TBil）、组织胺（HA）、干扰素γ（IFN Y）、白细胞介素12 （IL-12）、白细胞介素10 （IL-10）与白细胞介素4（IL-4）水平.用逆转录-多聚酶链反应（RT-PCR）方法观察肝组织IL-12 mRNA、IFN Y mRNA的表达水平.组间比较采用SPSS统计学软件进行单因素方差分析,进一步两两比较用SNK法进行统计学分析. 结果 （1）与正常对照组比较,实验性肝炎组ALT、TBil、血和肝组织HA水平均有明显升高（P＜ 0.05）,经抗组织胺治疗后,ALT、血和肝HA水平均明显降低（P＜0.05）.（2）实验性肝炎组IL-4、IL-10水平明显升高[（0.504±0.202）ng/ml、（29.025±1.478） pg/ml对比（0.811±0.244） ng/ml、（33.72±4.293） pg/ml,P＜0.05],而IL-12水平明显降低[（6.515±2.893） pg/ml对比（3.519±1.113） pg/ml,P＜0.05],经治疗后,IL-4、IL-10水平明显降低[分别为（0.423±0.168） ng/ml、（30.412±3.275） pg/ml,P＜0.05],IL-12水平明显升高（P＜0.05）,而IFN Y水平变化不明显（P＞0.05）.肝组织IL-12 mRNA与IFN Y mRNA水平也有同样的趋势.结论抗组织胺治疗可以明显改善肝功能,纠正Th1/Th2失衡.Objective To investigate the effects of antihistamine treatment on immune function in rats with experimental hepatitis.Methods Thirty Wistar rats were randomly allocated into three groups：experimental hepatitis group （EH group）,antihistamine treatment group （AH group） and normal control group （NC group）.Rats in the EH group received the subcutaneous injection of 40% carbon tetrachloride oil solution and were fed on diet with low-protein,low-choline,high-fat and highalcohol,while rats in the AH group received antihistamine treatment（ketotifen ＋ vitamin C） additionally.They were sacrificed after 4 weeks,and the levels of serum alanine aminotransferase（ALT）,total bilirubin （TBil）,histaminne（HA）,IFNγ,IL-12,IL-4 and IL-10 were determined.The levels of IL-12 mRNA and IFN-γmRNA in liver tissue were determined via real-time reverse transcriptional polymerase chain reaction（RT-PCR）.Results （1） Compared to the NC group,in the EH group,the levels of ALT,TBil,and circulating and intrahepatic HA were significantly increased（P 〈 0.05）; and the levels ofALT and circulating and intrahepatic HA were significantly decreased（P 〈 0.05） after antihistamine treatment.（2） Compared to the NC group,in the EH group,the levels of IL-4,IL-10 were significantly increased（（0.504 ± 0.202）ng/ml and （29.025 ± 1.478） pg/ml vs （0.811 ± 0.244）ng/ml and （33.72 ± 4.293）pg/ml respectively,P 〈 0.05）,and the levels of IL-12 were decreased （（6.515 ± 2.893）pg/ml vs （3.519 ± 1.113）pg/ml,P 〈 0.05）; and after antihistamine treatment the levels of IL-4 and IL-10 were significantly decreased （were （0.423 ± 0.168）ng/ml and （30.412 ± 3.275）pg/ml,P〈 0.05）,the levels of IL-12 were significantly increased （P 〈 0.05）,but the level of IFNγhad no siganificance （P 〉 0.05）.The levels of intrahepatic IL-12 mRNA and IFNγ mRNA had similar results.Conclusion Antihistamine treatment may improve liver function and correct Th1/Th2 unbalance.

关 键 词：肝炎 实验性 抗组织胺 TH1 TH2 

分 类 号：R575.1[医药卫生—消化系统]