凋亡细胞预处理对胰岛移植受体大鼠脾淋巴细胞功能的影响  

Effect of pretreatment with apoptotic donor spleen cells on spleen lymphocyte function of recipient rats after islet transplantation

作　　者：李双喜[1] 陈宏[1] 杨蕾[2] 陈容平[1] 张桦[1] 蔡德鸿[1] 张振[1] 

机构地区：[1]南方医科大学珠江医院内分泌科,广东广州510282 [2]南方医科大学珠江医院肾内科,广东广州510282

出　　处：《南方医科大学学报》2013年第10期1504-1507,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30470829)~~

摘　　要：目的研究凋亡细胞预处理对胰岛移植受体大鼠脾脏淋巴细胞功能的影响,探寻延长胰岛移植物存活时间的方法。方法无菌条件下获取供体的脾淋巴细胞悬液,采用60Coγ射线照射的方法诱导凋亡后,经尾静脉注射准备接受胰岛移植的糖尿病SD大鼠,1周后行肾包囊下原位胰岛移植术,在移植后的1周、2周以及发生排斥后利用2羧基荧光素二醋酸盐琥珀酰亚胺酯(CFSE)细胞染色法评估受体淋巴细胞的增殖反应能力,利用酶联免疫法(ELISA)检测受体脾脏淋巴细胞因子IL-2及IL-10水平。同时采用移植前注射生理盐水、供体的正常脾淋巴细胞及坏死脾淋巴细胞分别作为对照组。结果凋亡细胞预处理组的受体大鼠的淋巴细胞增殖指数及IL-2水平在移植前、移植后1周及2周均显著低于其他各组,而IL-10水平则明显高于其他各组,差异具有统计学意义(P<0.05)。结论凋亡细胞对受体脾淋巴细胞功能的这种作用可能是其参与移植免疫耐受形成的重要途径。Objective To study the effect of pretreatment with apoptotic donor spleen cells on spleen lymphocyte ffmction of recipient rats undergoing islet transplantation to explore new approaches to prolong islet graft survival. Methods Apoptotic spleen cells from donor rats were obtained by exposure to y-ray irradiation from 60Co. Diabetic SD rat models were randomly divided into 4 groups to receive tail vein injections with saline （group A）, normal cells （group B）, apoptotic donor cells （group C）, or necrotic donor cells （group D）. One week later, orthotopic transplantation of islets under the renal capsule was performed. Before and at I and 2 weeks after islet transplantation, the recipient rats were examined for proliferative activity of spleen lymphocytes with CFSE cell staining and for IL-2 and IL-10 expressions in the cells using ELISA. Results Pretreatment with donor apoptotic cells significantly suppressed the proliferative activity of recipient spleen lymphocytes before and at 1 and 2 weeks after islet transplantation as compared with the other three groups （P〈0.05）. The level of IL-2 was significantly decreased while IL-10 increased in apoptotic donor cell pretreatment group compared with those in the other 3 groups at each time point of observation. Conclusion The effect of pretreatment with apoptotic donor cells on recipient spleen lymphocytes suggest an important role of apoptotic donor spleen ceils in immune tolerance of grafts.

关 键 词：凋亡 胰岛移植 淋巴细胞增殖 淋巴细胞因子 

分 类 号：R363[医药卫生—病理学]