肿瘤抗原MAGE3预测CTL表位的合成与鉴定  

Synthesis and identification of predicted CTL epitopes derived from the tumor antigen MAGE 3

作　　者：贾正才[1] 吴玉章[1] 王祥智[1] 周伟[1] 

机构地区：[1]第三军医大学基础医学部全军免疫学研究所,重庆400038

出　　处：《第三军医大学学报》2000年第10期940-942,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 !(3990 0 1 34)

摘　　要：目的　合成并鉴定已预测出的 4个MAGE 3HLA A2限制性CTL表位多肽 ,为后续研究奠定基础。方法　采用固相合成法合成多肽 ,经RP HPLC纯化、纯度分析 ,用质谱进行定性鉴定。结果　 4个合成肽的纯度都在 95 %以上 ,经质谱分析 ,各肽的分子量测定值与理论值相符。结论　所得多肽为高纯度的目标肽 。Objective To synthesize and identify the four predicted HLA A2 restricted CTL epitope candidates from MAGE 3. Methods The peptides were synthesized with solid phase strategies, purified with reverse phase HPLC and identified with mass spectrometry. Their purity was determined too. Results All peptides were >95% in purity and the measured values of molecular weight were conformed to their theoretical values. Conclusion The collected products are the target peptides with high purity. This study provides reliable basis for further researches.

关 键 词：MAGE-3 固相合成 鉴定 肿瘤抗原 CTL表位 

分 类 号：R730.3[医药卫生—肿瘤] R392.11[医药卫生—临床医学]