IL-1预刺激对反义IRAK-2寡核苷酸抑制NF-κB活化的影响  

The effects of IL-1-pretreatment on inhibition of IL-1-induced NF-κB activation by antisense IRAK-2 oligonucleotide

作　　者：郭甫坤[1] 李亦蕾[1] 吴曙光[1] 

机构地区：[1]第一军医大学药物研究所,广东广州510515

出　　处：《第一军医大学学报》2000年第5期417-418,共2页Journal of First Military Medical University

摘　　要：目的 研究白介素－１（ＩＬ－１）预刺激对反义受体相关激酶－２寡核苷酸（ＩＲＡＫ－２ＯＤＮ）抑制ＩＬ－１激活核因子－ｋａｐｐａ Ｂ（ＮＦ－κＢ）的影响。方法 实验分ＩＬ－１预处理组和非预处理组。以脂质体介导反义ＩＲＫＡ－２ＯＤＮ转染２９３细胞，以夹心酶联免疫吸附测定法（ＥＬＩＳＡ）检测ＮＦ－κＢ的含量。结果 ＩＬ－１非预处理组反义ＩＲＡＫ－２ＯＤＮ不能抑制ＮＦ－κＢ活性。Objective To investigate the effects of IL-1-pretreatment on the inhibition of IL-1-induced NF-κB activation by antisense IL-1 kinase-2 receptor- associated (IRAK-2) oligonucleotide. Methods Antisense IRAK-2 oligonucleotide mediated by lipofectin encapsulation was used to transfect 293 cells. The levels of nuclear factor-kappa B(NF-κB) were determined by sandwich enzyme-linked immunosorbent assay. Results Antisense IRAK-2 oligonucleotide did not inhibit IL-1-induced NF-κB activation without IL-1-pretreatment. However, NF-κB activation was significantly inhibited by antisense IRAK-2 oligonucleotide in a time- (5~24 h) and dose- (1~8 (g) dependent manner if the cells were pretreated with IL-1. Conclusion IL-1-pretreatment is necessary for inhibition of IL-1-stimulated NF-κB activation by antisense IRAK-2 oligonucleotide.

关 键 词：预刺激 反义寡核苷酸 NF-ΚB IRAK-2ODN 

分 类 号：R346[医药卫生—基础医学]