p53、bcl-2及bax基因在非何杰金氏恶性淋巴瘤中的表达及意义  

Expression of p53、bcl-2 and bax genes in non-Hodgkin's lymphoma cells and its significance

作　　者：王立生[1] 赵俊生[2] 潘令嘉[1] 孙勇[1] 张亚历[1] 周殿元[1] 

机构地区：[1]第一军医大学全军消化内科研究所,广东广州510515 [2]兰州医学院病理学教研室,甘肃兰州730000

出　　处：《第一军医大学学报》2000年第5期422-424,共3页Journal of First Military Medical University

摘　　要：目的 探讨凋亡调控基因p53、bcl-2和bax的蛋白表达及其与非何杰金氏恶性淋巴瘤（NHL）恶性程度之间的关系。方法 运用免疫组化方法检测了62例NHL的凋亡调控基因p53、bcl-2和bax的蛋白表达状态。结果 p53及Bcl-2蛋白的表达率、表达强度及阳性细胞密度均随NHL恶性程度的发展而逐渐增高，低度恶性组明显低于中度及高度恶性组（P<0.01），而中度及高度恶性组之间则无显著差异（P>0.05）；与此相反，Bax蛋白在低度恶性组的表达水平明显高于中度及高度恶性组（P<0.01）。结论 细胞凋亡调控异常在NHL的发生与发展中可能起重要作用，它们的表达状态和NHL的恶性程度密切相关。Objective To explore the relationship between the expression of p53、bcl-2 and bax genes and the malignancy of non-Hodgkin's lymphomas. Method The expression of p53、bcl-2 and bax genes in non-Hodgkin's lymphoma cells from 62 cases was measured by employing immunohistochemical staining. Results The expression rate, expression intensity and positive cell density of p53 and Bcl-2 oncoproteins in non-Hodgkin's lymphomas increased gradually with the increment of tumor malignancy. It was significantly higher in low malignancy group compared with intermediate and high malignancy group (P<0.01). Obvious difference between intermediate and high malignancy group was not observed (P>0.05). In contrast, the expression level of Bax oncoprotein in low malignancy group was markedly higher than that in intermediate and high malignancy group (P<0.01).Conclusion Aberrant apoptosis regulation might play an important role in the occurrence and exacerbation of non-Hodgkin's lymphoma. There is significant correlation between the expression of these genes and the malignant degree of non-Hodgkin's lymphoma.

关 键 词：非何金氏恶性淋巴瘤 细胞凋亡 免疫组织化学 

分 类 号：R733.1[医药卫生—肿瘤]