阴阳莲提取物 3,4′,5-三羟基芪-3-单-D-葡萄糖苷对人大肠癌细胞系的体外效应  

In vitro effects of 3，4′，5-trihydroxystibene-3-β-mono- D-glucoside on human colorectal carcinoma cell lines

作　　者：李学农[1] 丁彦青[1] 周军[1] 朱梅刚[1] 

机构地区：[1]第一军医大学病理学教研室,广东广州510515

出　　处：《癌症》2000年第11期969-973,共5页Chinese Journal of Cancer

基　　金：国家自然科学基金! 39770912);军队医药卫生基金!(96Q47)资助

摘　　要：目的： 研究蓼科植物阴阳莲提取物 3， 4， 5-三羟基芪- 3-β-单- D-葡萄糖苷（ 3， 4， 5，- trihydroxystibene- 3-β- mono- D- glucoside， THMG）对大肠癌细胞的体外生长特性、粘附性及浸润力的影响；从细胞层次探讨 THMG抗转移作用的基本环节。方法：检测 4种大肠癌细胞（ HR8348， Hce8693， HT29， LoVo）在 THMG 0.4 mmol/L, 0.8 mmol/L, 1.6 mmol/L, 3.2 mmol/L等浓度作用下的存活率、生长曲线、粘附率及浸润力。结果：在实验浓度下， THMG对 4种大肠癌细胞的存活率、生长曲线无显著影响（ P >0.05），在 3.2 mmol/L浓度时对高转移性的 LoVo细胞的粘附抑制率为 68.09％ ,浸润抑制率为 89.95％ ,对低转移性的 HT29细胞粘附抑制率为 78.75％、浸润抑制率为 93.31％，且随着浓度加大，粘附和浸润抑制率提高。结论： THMG可能通过抑制癌细胞粘附性和浸润力，发挥抗转移效应。Objective： The current study was designed to investigate anti- adhesion and anti- invasion effects of 3， 4′， 5- trihydroxystibene- 3-β- mono- D- glucoside (THMG) on colorectal carcinoma cell lines in vitro. Methods： Four cell lines (HR8348, Hce8693, HT29 and LoVo) from human colorectal carcinoma were treated with 0.4 mmol/L, 0.8 mmol/L, 1.6 mmol/L and 3.2 mmol/L of THMG. Cell viability was assessed by trypanblue exclusion test. Adhesion test and quantitative invasion assay were performed by using modified human amniotic basement membrane (HABM)model and Boyden chamber model,respectively. Results： At the experimental concentration, THMG had no significant effects in terms of cell viability and cell growth curve on the colorectal carcinoma cell lines while at the concentration of 3.2 mmol/L THMG could markedly inhibit adhesion to HABM, with inhibit rate of 68.09％ for highly metastatic cell line(LoVo) and of 78.75％ for low metastatic cell line (HT29),respectively. Invasion inhibit rate determined by Matrigel invasion assay was 89.95％ for highly invasive cell line(LoVo), and 93.31％ for low invasive cell line(HT29), respectively. The adhesion inhibit rate and invasion inhibit rate were enhanced according to the concentration of THMG. Conclusion： THMG, an effective component from Chinese herb (polygonum cuspidatum sieb. et zucc), may have anti- metastasis effect through inhibit adhesiveness and invasiveness of colorectal carcinoma cells.

关 键 词：大肠肿瘤 THMG 肿瘤转移 细胞粘附 

分 类 号：R735.3[医药卫生—肿瘤]