清热解毒类与活血化瘀类中药对肺癌A549细胞增殖抑制的机制研究  

Chinese Medicine of Qingrejiedu and Huoxuehuayu the Mechanism of Inhibition of Proliferation of Lung Cancer A549 Cells

作　　者：周燕萍[1] 杨航[2] 关江峰[2] 周良发[2] 覃双来[2] 徐坚[2] 胡作为[2] 

机构地区：[1]湖北中医药大学,武汉430065 [2]湖北武汉市中西医结合医院肿瘤科,武汉430022

出　　处：《光明中医》2013年第10期2033-2036,共4页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：湖北省卫生厅青年科技人才基金(No:QJX2008 45)

摘　　要：目的探讨清热解毒类与活血化瘀类中药对肺癌A549细胞抑制增殖的可能机制。方法利用动物含药血清技术平台获得清热解毒类、活血化瘀类中药及正常血清。对数期A549细胞株随机分为3组(清热解毒药组、活血化瘀药组、正常血清组),将相对应的血清分别作用48h后,WB法检测CyclinD1、P53、PCNA、TNF-α、IL-1、CD4、CD8、BCL-2、Bax、VEGF,RT-PCR法检测BAX、bcl-2、VEGF,ELISA法检测TNF-α、IL-1。结果清热解毒药组与空白组相比,CyclinD1、PCNA表达明显下降(P<0.01),TNF-α、IL-1,CD4、CD8表达明显升高(P<0.01),CD4/CD8升高;P53、Bcl-2、Bax、VEGF无明显变化(P>0.05)。活血化瘀组与空白组相比,PCNA、BCL-2、VEGF表达明显下降(P<0.01),P53、Bax表达明显上升(P<0.01),CyclinD1、TNF-α、IL-1,CD4、CD8表达无明显差异(P>0.05)。结论清热解毒药与活血化瘀药既有共同的抗肿瘤机制,也有各自不同的抗肿瘤机制。清热解毒药的抗肿瘤机制体现在抑制增殖,诱生细胞因子,增强免疫活性方面,而活血化瘀类中药的抗肿瘤机制具体体现在抑制增殖,促进凋亡,抑制血管生成方面。Objective： Qingrejiedu and Huoxuehuayu Chinese medicine for lung cancer A549 cells to inhibit the proliferation of possible mechanisms. Methods the use of animal serum containing technology platform Qingrejiedu class, Chinese herbs of Huoxuehuayu and normal serum. Log phase A549 cells were randomly divided into 3 groups （ Qingrejiedu drug group, Huoxuehuayu drugs group, normal serum group） , and the corresponding serum respectively role after 48h WB detected expression of CyclinD1, P53, PCNA, TNF-α IL-1 , CD4, CD8, BCL-2, Bax, VEGF, RT-PCR method to detect BAX, bcl-2, of VEGF, ELISA assay of TNF-α, IL-1. Results Qingrejiedu drug group compared with the control group, CyclinD1, PCNA expression decreased significantly （P 〈 0.01 ）, TNF-α, IL-1, CD4, CD8 expression was significantly higher （ P 〈 0.01 ） , CD4/CD8 increased; P53, Bcl-2, Bax, VEGF, no significant change （ P 〉 0.05 ）. Huoxuehuayu group compared with the control group, PCNA, BCL-2, VEGF expression was significantly decreased （P 〈 0.01 ） , P53, Bax expression increased significantly （P 〈0. 01 ） , CyelinD1, TNF-α, IL-1, CD4, CD8 expression was no significant difference （P 〉0. 05）. Conclusion The heat antidote Huoxuehuayu drugs both anti-tumor mechanism, different antitumor mechanisms. Heat antidote to the antitumor mechanisms embodied in the inhibition of proliferation induced cytokines enhance immune activity, Huoxuehuayu Chinese herbs antitumor mechanism embodied in the inhibition of proliferation and promote apoptosis, inhibition of angiogenesis.

关 键 词：肺癌A549细胞 清热解毒 活血化瘀 抗肿瘤作用机制 

分 类 号：R734.2[医药卫生—肿瘤] R285.5[医药卫生—临床医学]