胰岛素对阿尔茨海黙病大鼠血浆血小板活化因子及海马突触可塑性的影响  

Effects of Insulin on the Platelet-activating Factor and Hippocampal Synaptic Plasticity of Rats Model with Alzheimer's Disease

作　　者：曾琳[1] 蒋政 谢明[1] 游咏[1] 

机构地区：[1]南华大学附属第一医院神经内科,湖南衡阳421001 [2]衡阳市第三人民医院内科,湖南衡阳421001

出　　处：《现代生物医学进展》2013年第25期4840-4842,4880,共4页Progress in Modern Biomedicine

基　　金：湖南省卫生厅项目(B2010-044)

摘　　要：目的:探讨胰岛素对阿尔茨海默病(AD)大鼠血浆血小板活化因子(PAF)及海马突触可塑性的影响。方法:将30只SD大鼠随机分为治疗组(10只)、模型组(10只)、假手术组(10只),采用侧脑室注射链脲霉素(STZ)建立AD大鼠模型。治疗组大鼠皮下注射胰岛素(0.1 U/kg),模型组及假手术组大鼠皮下注射等体积的生理盐水(1 mL/kg)。通过Morris's水迷宫实验评估各组大鼠的认知功能,酶联免疫吸附法测定各组大鼠的血浆PAF含量,免疫印迹法检测大鼠海马突触素的表达。结果:治疗4周后,模型组大鼠连续4天水迷宫的潜伏期均显著长于假手术组大鼠(P<0.05),而治疗组大鼠第2、3、4天水迷宫潜伏期均较模型组显著缩短(P<0.05),但仍长于假手术组大鼠(P<0.05);模型组大鼠的血浆PAF含量和海马突触素的表达均显著高于假手术组,而治疗组大鼠的血浆PAF含量和海马突触素的表达均显著低于模型组(P<0.05),差异均有统计学意义(P<0.05)。结论:皮下注射胰岛素可改善AD大鼠的认知功能,这可能与其下调AD大鼠血浆PAF水平以及保护突触的可塑性有关。Objective： To investigate the effects of insulin on the platelet=activating factor and hippocampal synaptic plasticity of rats with Alzheimer＇s disease （AD）. Methods： 30 Sprague-Dawley （SD） rats were randomly assigned to the treatment group （n=10）, AD model group（n=10） and sham group（n=10）. The rat models of AD were established by injecting Streptozotocin（STZ） intmcerebroventric= ularly. The rats in treatment group were hypodermically injected insulin （0.1U/kg）, the model group and the sham goup were hypodermi- cally injected wi.th saline （lmL/kg）. The cognitive ability were assessed by Morris＇s water maze test, the concentration of PAF was mea- sured by ELISA, western blot was used to detect the synaptophysin protein expression in hippocampus. Results： After 4 weeks＇ treatment, the latency in water maze test of rats in the model group were all significantly longer than those in the sham group for 4 days（P〈0.05）, while the latency in water maze test of rats in the treatment group on the 2nd, 3rd, 4th day were all significantly shorter than those in the model group（P〈0.05）; the plasma PAF level and protein expression of synaptophysin of rats in the model group were both markedly high- er than those in the sham group （P〈0.05）, but they were markedly lower in the treatment group than those in the model group（P〈0.05）. Conclusion： Hypodermic injection of insulin could improve the cognitive fimction of rats with AD, in which the down-regulation of plasma PAF level and protection of hippocampal synaptic plasticity may be involved.

关 键 词：胰岛素 阿尔茨海默病 血小板活化因子 海马突触素 可塑性 

分 类 号：R749.16[医药卫生—神经病学与精神病学]