肿瘤患者自体CD3AK与LAK细胞生物学特性研究  

Biological features of auto CD3AK and LAK induced from tumor patients

作　　者：徐琪[1] 安秀梅[1] 邵莹[1] 和世彦 郝希山[1] 

机构地区：[1]天津医科大学附属肿瘤医院天津市肿瘤研究所免疫室,天津300060

出　　处：《中国免疫学杂志》2000年第10期547-548,共2页Chinese Journal of Immunology

摘　　要：目的 :CD3AK细胞是由抗CD3单克隆抗体和IL 2共同激活诱导的肿瘤杀伤细胞。对 30例肿瘤患者自体CD3AK细胞及LAK细胞进行了比较性研究。方法 :采用MTT法检测CD3AK细胞及LAK细胞的杀瘤活性 ,应用流式细胞术进行免疫标志物分析。结果与结论 :肿瘤患者PBMNC诱导后产生的CD3AK细胞较LAK细胞有更强的增殖能力 ,但对Raji、K5 6 2细胞的生长抑制作用无明显区别 ;40 %～ 6 0 %CD3AK细胞表达CTL样表型 (CD3+ CD8+ ) ,约 40 %CD3AK细胞表达NK表型 (CD5 6 + )。同时亦观察到不同患者的免疫功能存在明显的个体差异。Objective: CD3AK cells were tumor-killing cells induced by costimulation of anti-CD3McAb and IL-2. We studied the difference between the auto CD3AK and LAK cells from 30 tumor patients.Methods:MTT colorimetric assay was used to show the cytotoxicity of CD3AK and LAK cells. The cell immunological characters were checked up by FCM.Results and Conclusions:CD3AK cells induced from tumor patients' PBMNC were more proliferous than LAK cells, and had no difference in cytotoxicity against Raji or K562. 40%～60% of CD3AK cells were phenotypes of CTL cells(CD3 +CD8 +),about 40% expressed phenotypes of NK cells(CD56 +). We also reported that there was some difference among tumor patients in immunological function.

关 键 词：CD3AK LAK 细胞 生物学特性 肿瘤 

分 类 号：R730.3[医药卫生—肿瘤] R392.12[医药卫生—临床医学]