护肝宁对CCl_4诱导肝细胞损伤保护作用的研究  

Protective Effect of Huganning Tablet on CCl_4 -Induced Liver Cell Injury in vivo and in vitro

作　　者：朱方石 吴晓燕[1] 丁永芳[1] 姚莉[2] 陆霜红[2] 

机构地区：[1]江苏省中医药研究院,江苏南京210028 [2]南京中医药大学附属中西医结合医院,江苏南京210028

出　　处：《辽宁中医杂志》2013年第10期1957-1959,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：南京中医药大学中医内科学教育部重点学科开放课题(023021024000-zynk004);江苏省科技创新与成果转化专项(BM2009903)

摘　　要：目的:探讨护肝宁对CCl4诱导肝细胞损伤的保护作用。方法:培养L-O2型肝细胞,12h后用CCl4体外诱导肝细胞损伤,分为正常对照组、模型对照组和护肝宁药液组,继续培养12 h,MTT检测各组细胞存活力;诱导肝细胞损伤模型制成后,再次分为CCl4模型组、正常血清对照组、护肝宁血清组,继续培养12 h,MTT检测各组细胞存活力。在复制肝损伤大鼠模型前,分正常对照组和模型对照组及护肝宁组3组,每组12只,连续7 d灌服相应液体,末次给药后6 h,模型对照组和护肝宁组腹腔注射50%CCl4花生油2 mL/kg,制作肝损伤大鼠模型,24 h后测定各组血清ALT,AST活性。结果:CCl4模型组细胞OD值较正常对照组降低(P<0.01),护肝宁药液组则明显高于CCl4模型组而低于正常对照组(P<0.05);护肝宁血清组细胞OD值则明显高于CCl4模型组和正常血清组(P<0.05);CCl4模型组及护肝宁组大鼠血清ALT、AST较正常组升高(P<0.01),但护肝宁组又较CCl4模型组为低(P<0.05)。结论:护肝宁具有抗肝细胞损伤和保护肝细胞的作用。Objective：To explore the protective effect of Huganning tablet on the CC14-induced hepatocyte injury animal mod- els. Methods：The cultured normal L-O2 hepatocytes and the carbon tetrachloride （CC14 ）-induced hepatocytes for 12 h in vitro were divided into normal control group, model control group and Huganning solution group. For the second 12 h, MTr assay was used to detect the cell viability in each group. At the same time, the model of injury hepatocytes were divided into CC14 model group, blank serum group, Huganning-containing serum group, and MTT assay was used to detect the cell viability in each group after the third 12 h. In addition,36 SD rats were evenly divided into three groups：normal control group, model control group, Hu- ganning solution group. Each group was ~iven appropriate liquid via gavaze once a day for consecutive 7 davs. Six hours after thelast administration, the rats of model control group and Huganning solution group received intraperitoneal injection of 50% CC14 peanut oil （2 mL/kg） in order to form liver injury model. Serum ALT and AST of all the rats in each group were detected after 24 h. Results ： The OD values of CC14 model group were lower than that of normal control group （ P 〈 0.01 ）, while that of Huganning solution group was significantly higher than that of CC14 model group but lower than that of normal serum group（ P 〈 0.05 ）. The OD values of Huganning-containing serum group were significantly higher than that of CC14 model group and blank serum group（ P 〈 0.05 ）. The rats＇ serum ALT and AST levels of model control group and Huganning solution group were higher than those of normal control group （ P 〈 0.01 ）. However, the rats＇ serum ALT and AST levels of Huganning solution group were lower than that of model control group（ P 〈 0.05 ）. Conclusion ： Huganning Tablet had effect of anti-liver cell damage and protecting liver cells.

关 键 词：护肝宁 含药血清 细胞培养 肝细胞 动物模型 抗肝损伤 

分 类 号：R-332[医药卫生]