川芎嗪对血虚证小鼠骨髓细胞IL-6和IL-18基因表达的影响  

Effect of Ligustrazine on IL - 6 and IL - 18 Gene Expression in Bone Marrow Cell of Blood Deficiency Mice Induced By Radiation

作　　者：郭平[1] 王升启[2] 

机构地区：[1]山东中医药大学基础医学院,山东济南250355 [2]军事医学科学院,北京100850

出　　处：《辽宁中医杂志》2013年第10期2152-2154,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(30271617)

摘　　要：目的:研究川芎嗪对辐射致血虚证小鼠骨髓细胞IL-6和IL-18基因表达的影响,为阐明川芎嗪补血和免疫调节作用的分子机制提供依据。方法:采用3.5 Gy60Coγ射线全身1次性照射制备小鼠血虚证模型;外周血白细胞计数;计算胸腺指数和脾指数;RT-PCR检测骨髓IL-6和IL-18 mRNA水平。结果:川芎嗪显著升高放射线致血虚证小鼠外周血白细胞数量(P<0.05)和脾指数(P<0.01),上调骨髓细胞IL-6(P<0.05)和IL-18(P<0.01)基因表达,对胸腺指数无显著影响。结论:川芎嗪促进骨髓细胞IL-6和IL-18基因的表达可能是其升血和调节免疫功能的机制之一。Objective：To study the effect of ligustrazine on IL -6 and IL - 18 gene expression in bone marrow cell of blood de- ficiency mice induced by radiation and provide the basis for clarifying the molecular mechanism of blood enriching and immuno- regulatory function of ligustrazine. Methods ：The blood deficiency mice were induced by using a single dose of 3.5 Gy 60 Co γ radi- ation. Peripheral blood leukocytes were counted and the thymus and spleen indexes were calculated. IL -6 and IL - 18 mRNA in bone marrow were measured by RT - PCR. Results ： Ligustrazine can significantly raise the peripheral blood leukocytes number （ P 〈0.05 ） and the spleen index （P 〈 0.01 ） of blood deficiency mice induced by radiation and up- regulate IL- 6 （P 〈 0.05 ） and IL - 18 （P 〈0.01 ） gene expressions of bone marrow cell of the mice,but had no significant effect on thymus index of the mice. Conclusion：Ligustrazine can promote IL- 6 and IL- 18 gene expression in bone marrow cells, which is probably one of the mechanisms of blood proliferation and immunoregulatory function of the ligustrazine.

关 键 词：川芎嗪 辐射 血虚证 白细胞介素 mRNA 

分 类 号：R-332[医药卫生]