SDF-1α/CXCR7促进BMSCs向缺血/再灌注大鼠海马CA1区迁移  

SDF-1/CXCR7 promotes the migration of BMSCs to ischemia/reperfusion hippocampal CA1 region of rats

作　　者：张世春[1,2] 何晓梅[1] 刘志安[1,3] 高殿帅[3] 徐铁军[3] 王玉兰[1,3] 

机构地区：[1]徐州医学院神经生物学实验室 [2]徐州市矿山医院,徐州221004 [3]徐州医学院解剖学教研室

出　　处：《神经解剖学杂志》2013年第5期513-518,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81171141);徐州医学院院长专项人才基金(2010KJZ19)

摘　　要：目的:研究基质细胞衍生因子-1α(stromal cell derived-factor-1α,SDF-1α)及其受体CXCR7在介导骨髓间充质干细胞(bone marrow derived mesenchymal stem cells,BMSCs)向大鼠脑缺血区迁移的作用。方法:BMSCs的原代培养、四血管阻断脑缺血模型制备大鼠脑缺血模型、侧脑室移植组(培养基干预组、BMSCs组和CXCR7抗体预处理BMSCs组)、免疫组织化学和免疫荧光组织化学染色方法。结果:免疫组织化学染色结果显示:在各组大鼠海马CA1区细胞内可见不同程度的呈黄色或棕黄色颗粒的SDF-1α免疫阳性产物,主要表达在细胞胞质中,CXCR7蛋白则主要表达在胞核和胞膜中;与假手术组比较,BMSCs组和CXCR7抗体预处理BMSCs组SDF-1α和CXCR7的表达均升高(P<0.05)。免疫荧光组织化学结果显示:侧脑室注射BMSCs并移植48 h后,BMSCs组、CXCR7抗体预处理BMSCs组海马CA1区均有不同程度荧光标记的阳性细胞;与BMSCs组比较,CXCR7抗体预处理BMSCs组阳性细胞数降低(P<0.05)。结论:缺血/再灌注损伤后,促进了SDF-1α及CXCR7阳性产物的表达;同时SDF-1α/CXCR7能够促进BMSCs向损伤区域的迁移。Objective： To study the role of stromal cell derived-fuctor-1α（SDF-1α） and its receptor CXCR7 in bone marrow derived mesenchymal stem ceils （BMSCs） migration to the cerebral isehemic area. Methods： The primary culture of BMSCs, the establishment of the four-vessel occlusion model of cerebral ischemia, intracerebroventricular transplanta- tion groups including cuhure medium interfering group; BMSCs group and CXCR7 antibody pretreated BMSCs group. The expression of SDF-1α and CXCR7 in in hippocampal CA1 of different groups were observed by immunohistochemical and immunofluorescent methods. Results： Immunohistochemistry results showed that some SDF-1α-immunopositive yellow or brown granule were mainly expressed in cell cytoplasm, and the expression of CXCR7-immunoreactivities were expressed in cell membrane and nuclei. Compared with sham-operated group, all SDF-1α and CXCR7 immunoreactive expression increased in BMSCs group and CXCR7 antibody pretreated BMSCs group （ P 〈 0.05 ）. hnmunofluorescent histochemistry results showed that after lateral ventricle injection transplantation for 48 hours, fluorescent cells were detected in hippocampal CA1 subfield in BMSCs group and CXCR7 antibody pretreated BMSCs group. The migrated fluorescent cells in CXCR7 antibody pretreated BMSCs group decreased when compared with BMSCs group （ P 〈 0.05 ）. Conclusion ： After ischemia-reperfusion injury, The expression of SDF-1α and CXCR7 increased in injury regions. SDF-1α/CXCR7 can pro- mote BMSCs migration towards injury regions.

关 键 词：基质细胞衍生因子-1Α CXCR7 CA1 骨髓间充质干细胞 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]