三氧化二砷抑制大鼠脑胶质瘤增殖  

Inhibition of 9L-induced glioma proliferation by intratumor administration of arsenic trioxide in rat

作　　者：崔爱玲[1] 郅颖[2] 李春玲[1] 赵松鹤[1] 贾阿林[1] 龚亮[1] 姜旭[1] 

机构地区：[1]新乡医学院河南高等学校组织再生重点开放实验室,新乡453003 [2]包头医学院附属医院检验科,包头014010

出　　处：《神经解剖学杂志》2013年第5期567-571,共5页Chinese Journal of Neuroanatomy

摘　　要：目的:观察三氧化二砷(As2O3)瘤内给药对大鼠脑胶质瘤的疗效,以探讨胶质瘤的有效化疗手段。方法:实验用65只(180±10)g SD大鼠,5只做正常对照,在60只大鼠纹状体部埋植导管,其中30只通过导管接种9L胶质瘤细胞株,建立在体脑胶质瘤模型。15只注入等量D-Hanks平衡盐,作为阴性对照;另15只为安查组,进行药物安全性研究。于植入瘤细胞第9 d,30只模型鼠随机分为肿瘤组与治疗组(各15只)。治疗组及安查组通过导管将As2O3(10μmol/L)注入瘤内。阴性对照组和肿瘤组注等量生理盐水。(1)阴性对照组、肿瘤组、安查组和治疗组于注射As2O38 d后各随机处死5只,迅速剥离脑组织,测量肿瘤体积,HE染色,观察组织形态变化,免疫组化检测PCNA含量。(2)阴性对照组、肿瘤组、安查组和治疗组各剩余的10只大鼠,逐日称量体重,观察其行为学变化,待其自然死亡后计算生存时间,并取脑,验证肿瘤的存在。结果:治疗组肿瘤明显小于肿瘤组,大鼠体重下降较肿瘤组为轻。肿瘤组平均生存期为25.8天,治疗组平均生存时间40天。但治疗组有两只未计入平均,一只生存178天,另一只在研究结束时被处死(达14个月),且处死后未发现肿瘤。结论:As2O3可通过减缓脑胶质瘤的增殖,缓解大鼠体重下降,延长模型动物生存时间,对大鼠脑胶质瘤具有明显治疗作用。Objective： To determine the possible efficacy of arsenic trioxide against malignant glioma in rat so as to give some clues to the clinical treatment. Methods： Sixty-five rats were used in the experiment. Five were raised as normal control. Catheters were implanted in the striatum of the rest 60 rats. Glioma cell line 9L was implanted via the catheter to induce glioma in 30 rats. Fifteen rats receiving eqnal volume of saline served as negative control. Another fifteen rats re- ceived only arsenic trioxide （As203 ） injection to investigate its safety. 30 model rats were then divided randomly into tumor and treatment groups （15 rats in each）. As2O3 （10 V1, 10 p, mol/L） was delivered into the tumor of the treatment group rats by catheter at 8 days after the implanting of 9L while the tumor group was given equal volume of saline. The rest 15 rats without 9L received equal volume of As2O3 and were used to check the safety. Five brains from each group were collected at 8 days after the administration of As2O3 to detect the size of tumor, observe tissue morphology after HE staining and evaluate PCNA level. For the rest 10 rats, eating, drinking, weight and life span （after 9L implantation） were recorded respectively. Brains were taken out after death to check the existence of tumor. Results ： 9L induced tumor arise in the brain of the rats. The proliferation of the tumor was slowed down by As2O3 significantly compared with thetumor group （P 〈 0.05 ）. AszO3 attenuated 9L-induced decrease of the weight and prolonged life span of the model rats. The averaged life span of the tumor group was 25.8 days while the treatment group was 40 days. Two rats of the treatment group lived much longer and were excluded for the statistieal analysis, one lived 178 days; the other was killed at the end of the experiment and lived more than 14 months. Tumor group had more cells with alien nuclear while treatment group had less. PCNA was expressed higher in tumor group than that in the treatment group （ P 〈 0.0

关 键 词：胶质瘤 AS2O3 化疗 瘤内给药 大鼠 

分 类 号：R739.4[医药卫生—肿瘤]