线栓法大鼠大脑中动脉脑缺血模型的评价方法初探  

Preliminary Study for Evaluation System of Rat Permanent Cerebral Artery Occlusion Model by Intraluminal Suture Method

作　　者：邢凤英[1] 周颖[1] 马政文[1] 吴文庆[2] 李垚[1] 陈学进[1] 严国锋[1] 

机构地区：[1]上海交通大学医学院实验动物科学部,上海200025 [2]上海交通大学生物医学工程,上海200240

出　　处：《实验动物与比较医学》2013年第5期339-346,共8页Laboratory Animal and Comparative Medicine

基　　金：上海市科学技术委员会资助项目（071409010）

摘　　要：目的 初步探讨及完善线栓法大鼠中动脉脑缺血模型的评价体系.方法 将自制线栓从颈内动脉插入大鼠大脑中动脉并闭合创口,制备永久性大鼠脑缺血模型,模型制备前称其体质量,脑缺血24 h后分别称取体质量,进行神经行为学评分、脑电波检测、TTC染色、HE染色和脑水分测定.结果 根据修改后的13分评分标准,对脑缺血24 h后的大鼠作神经行为评价并进行分组,雄性大鼠5分以下（包括5分）和5分以上组在体质量变化均存在明显的差异（P＜0.05）,而雌性大鼠两组间没有明显差异.本实验选取5分以上组做进一步的评价,发现雄性大鼠缺血侧的脑含水比显著高于雌性大鼠（P＜0.01）.梗死面积雄性大鼠略大于雌性,但二者之间无显著性差异.HE染色发现5分以上组右脑比正常大鼠存在明显的结构破坏,脑电分析也表明手术后的右脑明显比左脑的输出输入信息受阻明显.结论 经过TTC、HE和脑电等的评价方式,对采用13分制神经行为学评分以5分作分界提供了实验依据,确认了只有达到5分值以上的模型才是成功及有效的脑缺血模型.Objective To explore the evaluation system of rat permanent cerebral artery occlusion （MCAO） model.Methods A middle cerebral artery occluded by inserting a nylon monofilament via the right external carotid artery to produce rat permanent MCAO model.The rats were weighed before the surgery and 24 h following ischemia.Neurological behavior evaluation,brain wave detection,TTC staining,HE staining and brain water content determination were evaluated in all surviving rats 24 h following ischemia as well.Results The neurological behavior evaluation of MCAO rats 24 h following ischemia were assessed according to modified 13-point score standard,and then grouping.There is significant difference in male rats weight between score 5-below （including score 5） group and score 5-above group （P〈0.05）.However,there is no difference of female rats between those 2 groups.Then score 5-above group was further evaluated,and found that male rats brain water content ratio of ischemic side was significantly higher than that of female rats （P〈0.01）,the infarction area of male rat was wider than that of female rats but there is no statistic difference.The result of HE staining showed that the right brain structure of score 5-above group was damaged obviously compare to normal brain.The result of EEG analysis also showed that the input and output information of right ischemic side of brain was hindered significantly compare to the left normal side.Conclusions TTC,HE,EEG evaluations provide the experimental evidence for using score 5 points as a boundary according to 13-point score standard.The data also confirmed that the cerebral ischemia model which neurological score only above 5 points is a successful and effective rat MCAO model.

关 键 词：线栓法 中动脉闭塞脑缺血 评价体系 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]