宫颈腺癌组织ERK1/2和p16^(INK4a)及P-gp表达与耐药相关性分析  

Expressions of ERK1/2,p16^(INK4a) and P-gp in cervical adenocarcinoma and its relation to chemoresistance

作　　者：曾四元[1,2] 江维[2] 梁美蓉[2] 李凌[2] 钟美玲[2] 

机构地区：[1]南昌大学医学院,江西南昌330006 [2]江西省妇幼保健院肿瘤科,江西南昌330006

出　　处：《中华肿瘤防治杂志》2013年第19期1508-1512,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(81260383)

摘　　要：目的:探讨细胞外信号调节激酶1/2(ERK1/2)、p16INK4a及P-糖蛋白(P-gp)与宫颈腺癌耐药的关系。方法:PV9000免疫组化二步法检测化疗敏感组(14例)及耐药组(25例)宫颈腺癌活检组织中ERK1/2、p16INK4a及P-gp蛋白的表达情况,分析ERK1/2、p16INK4a及P-gp在两组的表达差异、3者的表达相关性及与宫颈腺癌患者3年生存率的关系。结果:ERK1/2在耐药组及敏感组的高表达率分别为72.0%(18/25)及35.7%(5/14),差异有统计学意义,U=96,P=0.016;P-gp在耐药组的高表达率为76.0%(19/25),显著高于敏感组的28.6%(4/14),差异有统计学意义,U=102,P=0.026;p16INK4a蛋白在耐药组及敏感组的表达差异无统计学意义,U=129,P=0.140。ERK1/2与p16INK4a的表达呈正相关,r=0.571,P<0.001;ERK1/2与P-gp的表达呈正相关,r=0.364,P=0.023;p16INK4a与P-gp的表达无明显相关性,r=0.254,P=0.132。ERK1/2高表达与低表达患者的3年生存率分别为60.9%(14/23)和93.8%(15/16),差异有统计学意义,χ2=4.723,P=0.030;而p16INK4a高表达与低表达患者的3年生存率分别为68.0%(17/25)和85.7%(12/14),差异无统计学意义,χ2=1.355,P=0.244;P-gp高表达与低表达患者的3年生存率分别为65.2%(15/23)和87.5%(14/16),差异无统计学意义,χ2=2.347,P=0.125。结论:ERK1/2和P-gp的高表达与宫颈腺癌耐药相关;ERK1/2的表达情况不仅可为宫颈腺癌化疗用药提供参考,而且可作为宫颈腺癌判断预后的指标。OBJECTIVE：To explore relation between the expressions of extracellular signal-regulated kinase 1/2（ERK1/2）,p16INK4a,P-glycoprotein（P-gp）and the chemoresistance of cervical adenocarcinoma.METHODS：PV9000immunohistochemistry was used to detect the expressions of ERK1/2,p16INK4aand P-gp in the biopsy specimens of cervical adenocarcinoma of chemosensitive group（14patients）and chemoresistant group（25patients）.Expression differences of ERK1/2,p16INK4aand P-gp between the chemosensitive group and chemoresistant group and the correlation among them were analyzed.The relation between the expressions of ERK1/2,p16INK4aand P-gp and the 3-year survival rate of the patients with cervical adenocarcinoma was analyzed.RESULTS：The highly expressed rates of ERK1/2in chemoresistant group and chemosensitive group were 72.0%（18/25）and 35.7%（5/14）respectively,and there was statistical significance between them（U=96,P=0.016）;the highly expressed rate of P-gp was 76.0%（19/25）in chemoresistant group which was obviously higher than that of chemosensitive group 28.6%（4/14）,and this difference was statistically significant（U=102,P=0.026）;while the expression differences of p16INK4abetween the chemoresistant group and chemosensitive group were not statistically significant（U =129,P=0.140）.The expression of ERK 1/2was positively correlated with that of p16INk4a（r=0.571,P0.001）,and the expression of ERK1/2was positively correlated with that of P-gp（r=0.364,P=0.023）,while there was no obvious correlation between the expression of p16INK4aand the expression of P-gp（r=0.254,P=0.132）.The 3-year survival rates of ERK1/2highly expressed patients and lowly expressed patients were60.9%（14/23）and 93.8%（15/16）respectively,and there was statistically significant between them（χ2=4.723,P=0.030）;while the 3-year survival rates of p16INK4ahighly expressed patients and lowly expressed patients were 68.0%（17/25）and 85.7%（12/14）,there was no statistical significance between them�

关 键 词：宫颈肿瘤 ERK1 2蛋白 P16INK4A蛋白 P-糖蛋白 抗药性 多药 

分 类 号：R737.33[医药卫生—肿瘤]