在大鼠牙髓炎中的八聚体结合转录因子4B免疫组化研究  

An Experimental Study on the Dynamic Expression of Oct-4B in Rats with Pulpitis by Immunohistochemistry

作　　者：兰卫东[1] 董迎春[1] 

机构地区：[1]南京大学附属口腔医院,江苏南京230001

出　　处：《昆明医科大学学报》2013年第10期10-13,21,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81100768)

摘　　要：目的研究内毒素(LPS)诱导大鼠牙髓炎模型中八聚体结合转录因子4B(Oct-4B)在不同时间的表达特点及定位情况,以探讨Oct-4B在牙髓炎症中的可能作用,推测它在牙髓炎发生、发展中的意义.方法通过对LPS诱导的大鼠磨牙牙髓炎进行连续组织切片,同时采用免疫组织化学检测大鼠牙髓炎中Oct-4B的表达情况.结果 1 d组:成牙本质细胞呈中度阳性表达,牙髓成纤维细胞为中度至强阳性表达;3、5、7 d组:成牙本质细胞、牙髓成纤维细胞阳性表达减弱.2周组:坏死区扩大呈均质弱阳性表达,成牙本质细胞、牙髓成维维细胞及内皮细胞减少,呈阳性,修复牙本质呈弱阳性表达;3周组:大部分坏死牙髓组织呈均质弱阳性表达.结论 Oct-4B在大鼠牙髓炎发生、发展呈动态表达,Oct-4B可能参与调节牙髓炎发生、发展及修复过程.Objective To establish a model of rat endotoxic pulpitis induced by Lipopolysaccharide （LPS）;to study the dynamic expression and location of Oct-4B in rat pulpitis model at different time;To evaluate the role of Oct-4B on pulp injury and reparation in rat endotoxic pulpitis. Method The slides were made and the dynamic expression and location of Oct-4B was examined by immunohistochemistry. Results Oct-4B was moderately positive in the pulp fibroblasts and odontoblasts in 2 weeks. After 2 weeks, it was still positive in odontoblasts and fibroblasts. Up to 3 weeks, it was weakly positive in odontoblasts and fibroblasts. Conclusion Oct-4B is dynamically expressed in the rats with pulpitis, and may play an important role in the incidence,development and recovery of pulpitis.

关 键 词：Oct-4B 牙髓炎 大鼠 

分 类 号：R781.3[医药卫生—口腔医学]