沙丁胺醇在3,4-二氯苯基异氰酸酯万古霉素手性柱上的对映体分离  

Enantioseparation of Salbutamol on the 3,4-Dichlorophenyl Isocyanate Vancomycin Chiral Stationary Phase

作　　者：于萍 苏蕾 刘光[3] 李丽[4] 

机构地区：[1]云南省食品药品监督管理局不良反应中心,云南昆明650041 [2]普洱市第一人民医院药剂科,云南普洱665000 [3]昆明医科大学药学院,云南昆明650500 [4]云南省肿瘤医院药剂科,云南昆明650052

出　　处：《昆明医科大学学报》2013年第10期39-41,共3页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金资助项目(09Z0036)

摘　　要：目的建立一种在自制手性柱上对沙丁胺醇进行了对映体分离的方法.方法利用极性流动相中不同浓度的酸、碱添加剂在自制的3,4-二氯苯基异氰酸酯万古霉素手性柱对沙丁胺醇对映体分离的研究,初步探讨了手性识别机理.结果流动相中酸、碱添加剂的比例为0.01%:0.01%(V/V),流速为1 mL/min,柱温为25℃时,沙丁胺醇获得了最好的对映体分离,选择因子为1.16,分离度达到1.41.结论自制的3,4-二氯苯基异氰酸酯万古霉素手性柱对沙丁胺醇有一定的分离效果,可以此为参考,开发其他类似的手性固定相.Objective To establish a self-made chiral column for enantiomeric separation of salbutamol. Methods We used different concentrations of acid,alkali additives in the polar phase flow for enantiomers separation of salbutamol by using 3, 4-dichlorophenyl isocyanate vancomycin chiral column, and discussed the chiral recognition mechanism. Results The ratio of acid to alkali additive in the mobile phase was 0.01%：0.01% （V/V）, the flow rate was 1ml/min, the column temperature was 25℃, and the best separation of enantiomers of salbutamol was obtained,the selective factor was 1.16, the separation degree reached 1.41. Conclusion Self-made 3, 4-two chlorophenyl isocyanate vancomycin chiral column is effective for salbutamol separation, and it can be as a reference for developing other similar chiral stationary phase.

关 键 词：对映体分离 沙丁胺醇 极性流动相 手性识别机理 

分 类 号：R962[医药卫生—药理学]