颅咽管瘤中RARα及PPARβ/δ表达与RA作用的相关性  

Correlation between the Expression of RARα, PPARβ/δ and the Effect of Retinoic Acid in Craniopharyngioma Cells

作　　者：罗林[1] 白刚[1] 王行桥 倪炜[1] 左频[1] 袁红平[1] 牛华涛[1] 范耀东[1] 

机构地区：[1]云南省肿瘤医院神经外科,云南昆明650118 [2]山东省威海卫人民医院神经外科,山东威海264200

出　　处：《昆明医科大学学报》2013年第10期42-46,共5页Journal of Kunming Medical University

基　　金：云南省科技厅专项研究基金资助项目(2008CD038)

摘　　要：目的检测颅咽管瘤中RARα及PPARβ/δ的表达,分析其表达量与RA作用效果的相关性,探讨RA靶向治疗颅咽管瘤的分子机制.方法 RT-PCR法检测31例体外培养的颅咽管瘤细胞中PPARβ/δ、RARα的表达情况,MTT法测定RA对PPARβ/δ、RARα不同表达的颅咽管瘤细胞的抑制率,分析其表达情况与RA作用是否存在相关性.结果 (1)RT-PCR结果显示,不同颅咽管瘤细胞中PPARβ/δ、RARα表达存在差别.31例原代培养的颅咽管瘤细胞按其核受体表达不同分为PPARβ/δ>RARα、RARα>PPARβ/δ、RARα>>PPARβ/δ3组;(2)MTT结果显示在相同RA药物作用下RARα>>PPARβ/δ组的抑制率明显高于RARα>PPARβ/δ组、PPARβ/δ>RARα和对照组,差异有统计学意义(P<0.05).结论 (1)PPARβ/δ、RARα的表达可作为评估RA治疗颅咽管瘤效果的有效指标.PPARβ/δ表达低的颅咽管瘤细胞对RA更敏感;(2)靶向升高RARα或者靶向降低PPARβ/δ的表达都有益于颅咽管瘤的治疗.Objective To investigate the molecular mechanism of retinoic acid in targeted treatment of craniopharyngioma by detecting the expression of RARαand PPARβ/δin craniopharyngioma cells and analyzing the correlation between the expression and effect of retinoic acid. Methods The expression of RARα and PPARβ/δ in craniopharyngioma cells from 31 patients cultured in vitro was quantified by reverse transcription-PCR. The inhibition rates of RA on craniopharyngioma with different expression of RARα and PPARβ/δ were detected by using MTT assay, and the correlation between the expression of RARα and PPARβ/δand the effect of RA was analyzed. Results 1. The RT-PCR results showed that the expression levels of PPARβ/δand RARα mRNA were different. Craniopharyngioma cells from 31 patients in primary culture were divided into three groups according the expression levels of nuclear receptor： PPARβ/δ〉RARα group, RARα〉PPARβ/δ group and RARα〉〉PPARβ/δ group. 2.MTT results showed that the inhibition rate of RARα〉〉PPARβ/δgroup was significantly higher than the other groups under same drug, the differences had statistical significance （ 〈0.01） . Conclusions The expression of PPARβ/δ, RARα can be used to evaluate the effect of RA in treatment of craniopharyngioma. The craniopharyngioma with low-expression of PPARβ/δ is more sensitive to RA. Targeting higher RARα or targeting lower PPARβ/δ is beneficial to the treatment of craniopharyngiomas.

关 键 词：过氧化物酶体增殖物活化受体 β δ核维甲酸受体 颅咽管瘤 

分 类 号：R739.41[医药卫生—肿瘤]