miR-26b过表达对不同时间点人脂肪细胞分泌脂因子的影响  

Secretion of adipokines in miR-26b over-expressed human adipocytes differentiation process

作　　者：史春梅[1,2] 徐广峰[3] 季晨博[1,2] 陈玲[1,2] 杨蕾[1,2] 庞玲霞[1,2] 赵亚萍[3] 郭锡熔[1,2] 

机构地区：[1]南京医科大学附属南京妇幼保健院儿童保健科,江苏南京210004 [2]南京医科大学儿科研究所,江苏南京210029 [3]中国人民解放军第82医院检验科,江苏淮安223001

出　　处：《临床儿科杂志》2013年第10期914-916,共3页Journal of Clinical Pediatrics

基　　金：国家重点基础研究发展计划(No.2013CB530604);国家自然科学基金(No.81170797);江苏省自然科学基金(No.BK2011107);江苏省医学创新团队项目(No.LJ201108);南京市科技发展计划(No.201104013);蚌埠医学院科研课题项目(No.Byky1262NF);军区医学科技创新课题项目(No.12MA025)

摘　　要：目的探讨miR-26b过表达在人脂肪细胞分化过程中对各种脂因子分泌的影响。方法以miR-26b过表达病毒感染人脂肪前体细胞并诱导分化;收集不同时间点的细胞培养基上清,采用酶联免疫吸附法(ELISA)检测各个时间点的脂因子(白介素-6、瘦素、抵抗素、肿瘤坏死因子-α)分泌量。结果与对照组比较,在miR-26b过表达病毒感染的人脂肪前体细胞分化过程中,白介素-6、瘦素分泌量降低,而抵抗素分泌量升高;两组均未检测到肿瘤坏死因子-α。结论 miR-26b在一定程度上可以改善人脂肪细胞炎症状态及改善胰岛素抵抗,为肥胖治疗提供潜在靶点。Objective To investigate the influence of overexpression of miR-26b on the secretion of adipokines dur- ing human adipocyte differentiation. Methods Human preadipocytes were infected with the hsa-miR-26b over-expressing lentivirus and were induced to differentiate, and then the levels of adipokines （IL-6, leptin, resistin, TNF-α） at different time points during differentiation were measured by ELISA. Results Compared with control group, decreased secretions of both IL-6 and leptin, and increased secretion of resistin were found during the differentiation of human adipocytes in miR- 26b overexpressed group. However, the secretion of TNF-a was not measured in both groups. Conclusion The miR-26b can improve the inflammation and insulin resistance of human adipocytes, which will provide potential targets for obesity treatment.

关 键 词：miR-26b 脂因子 肥胖 人脂肪细胞 

分 类 号：R363[医药卫生—病理学]