骨桥蛋白和原癌基因c-Met在胃癌中的表达及其临床意义  

Expressions and significances of osteopontin and c-Metin gastric cancer

作　　者：马桂芳[1] 蔡红星[1] 丁凤云[1] 刘冉[2] 李云晖[2] 

机构地区：[1]盐城卫生职业技术学院检验教研室,江苏盐城224006 [2]东南大学公共卫生学院教育部环境医学工程重点实验室,江苏南京210009

出　　处：《癌变．畸变．突变》2013年第5期352-356,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(81172747);江苏省自然科学基金(BK2010407);盐城市医学科技发展计划项目(YK2011129)

摘　　要：目的:研究骨桥蛋白(osteopontin,OPN)和原癌基因c-Met在胃癌组织中的表达及其临床意义。方法:用免疫组织化学SP法检测69例胃癌组织、20例胃黏膜不典型增生组织及20例正常胃黏膜组织中OPN蛋白和c-Met蛋白的表达水平,并探讨其表达与肿瘤临床病理指标的关系;应用实时荧光定量PCR方法检测15例胃癌和正常切缘组织(距肿瘤边缘5 cm以上)中的OPN mRNA和cMet mRNA的表达。结果:OPN蛋白在胃癌组织中的阳性表达率为69.6%,高于胃黏膜不典型增生组织(40.0%)及正常胃黏膜组织(30.0%)(P<0.05);OPN蛋白表达与肿瘤的浸润深度、临床TNM分期、淋巴结转移之间有明显的相关性;胃癌组织和正常胃黏膜组织中OPN mRNA表达水平分别为β-actin的2.08和0.46倍,胃癌组织明显高于正常胃黏膜组织(P<0.01)。c-Met蛋白在胃癌组织中的阳性表达率为65.2%,明显高于胃黏膜不典型增生组织(30.0%)及正常胃黏膜组织(20.0%)(P<0.01);c-Met蛋白表达水平与肿瘤的淋巴结转移、临床TNM分期之间有明显的相关性;胃癌组织和正常胃黏膜组织中c-Met mRNA表达水平分别是β-actin的0.21和0.03倍,胃癌组织明显高于正常胃黏膜组织(P<0.01)。结论:OPN、c-Met高表达可能促进了胃癌的发病过程,在胃癌的发生发展过程中具有重要作用。OBJECTIVE： To investigate the relationship between the expressions of osteopontin（OPN） and c- Met and its clinical significance in gastric cancer tissue. METHODS： Immunohistochemical method was used to detect the expression levels of OPN protein and c-Met protein in 69 tissue specimens from patients with gastric cancer, 20 cases with nontypical hyperplasia, and 20 normal gastric mucosa specimens. We also investigated the relationships between their expressions and clinicopathological parameters; real-time quantitative reverse transcription-polymerase chain reaction（real-time PCR） was performed in 15 fresh gastric cancer samples and the corresponding normal tissue to detect mRNA expressions of OPN gene and c-Met gene. RESULTS： The positive rate of OPN was 69.6% in gastric cancer, which was significantly higher than 40.0% in nontypical hyperplasia tissues and 30.0% in normal gastric mucosa （P〈0.05）. The expression of OPN protein was closely correlated to the depth of invasion, TNM stage and lymph node metastasis. The expressions of OPN gene in gastric cancer tissues and normal gastric mueosa specimens were 2.08 and 0.46 times, respctively. The expression of OPN gene in gastric cancer tissues was significantly higher than normal gastric mucosa specimens （P〈0.01）. The positive rate of c-Met was 65.2% in gastric cancer, which was significantly higher than 30.0% in nontypical hyperplasia tissues and 20.0% in normal gastric mucosa （P〈0.01）. The expression of c-Met protein was closely correlated to the lymph node metastasis and TNM stage. The expressions of c-Met gene in gastric cancer tissuesand normal gastric cancer tissues was expressions of OPN mucosa specimens were 0.21 and 0.03 times, respectively. The expression of c-Met gene significantly higher than normal gastric and c-Met may play important roles in the mucosa specimens （P〈0.01）. CONCLUSION： pathogenesis and progression of gastric cancer. in gastric Increased

关 键 词：胃癌 转移 骨桥蛋白 c—Met 

分 类 号：R735.2[医药卫生—肿瘤]