新疆哈萨克族和汉族食管癌患者金属硫蛋白-3CpG岛甲基化的研究  

Methylation of the CpG island in MT-3 gene in esophageal carcinoma in Kazaks and Hans in Xinjiang

作　　者：田薇[1] 张慧霞[1] 陈艳[1] 

机构地区：[1]新疆医科大学公共卫生学院,新疆乌鲁木齐830011

出　　处：《癌变．畸变．突变》2013年第5期357-359,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：新疆医科大学创新基金(XJC201083)

摘　　要：目的:检测分析和比较新疆哈萨克族和汉族食管癌及癌旁组织中金属硫蛋白-3(metallothionein-3,MT-3)基因启动区CpG岛的甲基化情况,为寻找新疆哈萨克族食管癌诊断治疗的分子标记物提供线索。方法:采用甲基化特异性聚合酶链反应(methylation specific polymerase chain reaction,MSP)的方法,检测33例哈萨克族和30例汉族食管癌患者食管癌组织和癌旁组织中MT-3基因启动子区CpG岛的甲基化发生状况。结果:33例哈萨克族食管癌组织和癌旁组织标本中MT-3基因启动子区甲基化发生率分别2为66.7%(22/33)和27.3%(9/33),差异有统计学意义(χ=10.280,P=0.001);30例汉族食管癌组织和癌旁组织标本中MT-3基因启动子2区甲基化发生率分别为56.7%(17/30)和26.7%(8/30),差异亦有统计学意义(χ=5.554,P=0.018)。哈、汉民族之间癌组织和癌旁组2 2织MT-3基因启动子区甲基化率的差异均无统计学意义(χ=0.666,P=0.414;χ=0.003,P=0.957)。结论:MT-3基因启动子区CpG岛的甲基化可能与食管癌的发生与发展有关,但不是哈族食管癌患者的特异性致病因素。OBJECTIVE： To explore the relationship between the level of Methylation of the CpG island in the MT-3 gene in esophageal carcinoma in Kazaks and Hans ethnic groups. METHODS： The methylation of MT-3 gene was assessed by methylation specific polymerase chain reaction （MSP） in esophageal cancer tissues and adjacent tissues. RESULTS ： The methylation rates of the MT-3 gene in esophageal cancer tissues and adjacent tissues were 66.7% （22/33） and 27.3% （9/33）, respectively in Kazaks, 56.7% （17/30） and 26.7% （8/30）, respectively in Hans. Significant differences were seen in the methylation rates of MT-3 gene in esophageal cancer and adjacent tissues（ X 2=10-280, P=0. 001; X 2=5.554, P=0.018）. But there were no significant differences in the methylation rates in esophageal cancer and adjacent tissues between Kazaks and Hans. CONCLUSION： Aberrant promoter methylation of the CpG island in MT-3 gene may play an important role in the pathogenesis of esophageal squamous cell carcinoma, but it may not be a specific risk factor for esophageal cancer in the Kazaks.

关 键 词：哈萨克族 汉族 食管癌 金属硫蛋白-3 甲基化特异性聚合酶链反应 

分 类 号：R735.1[医药卫生—肿瘤]