KLK5基因在基底细胞样乳腺癌组织中的差异表达  

Different expression of KLK5 genes in basal-like breast cancer

作　　者：吴燕梅[1] 郑唯强[2] 刘夕水[1] 李曦洲[1] 吴凯男[1] 施俊义[1] 

机构地区：[1]第二军医大学长海医院甲乳外科,上海200433 [2]第二军医大学长海医院病理科,上海200433

出　　处：《中国现代普通外科进展》2013年第9期673-677,共5页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金(30870975;81272905)

摘　　要：目的:探索KLK5基因作为诊断基底细胞样乳腺癌(BLBC)的分子标志和治疗靶点的可能。方法:通过包含48 804个探针的人类mRNA基因表达谱芯片比较48例各亚型乳腺癌和6例正常乳腺组织基因表达谱,并通过荧光实时定量PCR法进行结果验证,分析BLBC与其他各亚型乳腺癌基因之间的差异表达。结果:分析乳腺癌各亚型和正常乳腺组织的基因表达谱,在BLBC中有99个基因上调4倍以上(2倍以上有意义),其中KLK5基因显著上调(5倍以上),但KLK5在Luminal A、Luminal B、HER-2过表达的基因表达谱中下调超过2倍,它们之间的差异表达具有统计学意义(P<0.05);并通过RT-PCR检测得到进一步验证。结论:KLK5可以作为诊断基底细胞样乳腺癌的标志基因和治疗靶点。Objective： To investigate the underlying role of gene KLK5 as the therapeutic tar- gets for basal-like breast carcinoma. Methods： The gene-expression profiles of 48 cases of the breast carcinoma and 6 cases of normal mammary epithelium were analysed using human mRNA genome expression profiling chip containing 48 804 probes in attempt to characterize molecular mechanism involved in the carcinogenesis of basal-like carcinoma of the breast, Real time-PCR was also used to validate results of each of the breast carcinoma subtype tissues and normal mammary epithelium tissues. Reults： The mRNA gene expression profiling analysis of breast cancer mRNA and normal mammary ductal epithelial cells genomes expression profiling chips, it showed that 99 genes in BLBC upregulates more than 4-folds （more than 2-folds are indentified as significant）, of these the gene KLK5 upregulates significantly （more than 5-folds）.However, the gene KLK5 in Lu- minal A,Luminal B and HER2-positive breast cancer cells downregulates more than 2-folds, the dif- ferences have statistic significance （P〈O.05）. RT-PCR and signaling network were further analyzed for the molecular regulation.The result is the same. Conclusion： It was found gene KLK5 in BLBC was significantly differed with other subtypes, such result has not been reported. It was assumed that KLK5 diferentially expressed gene could be used as a novel marker and a therapeutic target for BLBC.

关 键 词：KLK5 乳腺肿瘤 MICRORNA 差异表达 

分 类 号：R737.9[医药卫生—肿瘤]