结直肠腺癌发病基因fbxl20和set的相关性研究  

Correlation research about the colorectal adenocarcinoma gene fbxl20 and set

作　　者：朱晓华[1] 谢杰斌[2] 邓世山[1] 谢兴国[1] 荣欣欣[1] 燕芳[3] 朱剑军[1] 

机构地区：[1]川北医学院人体解剖学教研室,四川南充637000 [2]川北医学院附属医院普外科,四川南充637000 [3]重庆大学生命科学院基因工程研究中心,重庆400030

出　　处：《川北医学院学报》2013年第5期451-456,共6页Journal of North Sichuan Medical College

基　　金：四川省教育厅基金项目(13ZB0242);四川省科技厅项目(2012ZZ008);川北医学院科研发展计划项目(CBY12-A-QA01)

摘　　要：目的:通过半定量PCR技术,研究fbxl20基因与set基因与临床病理特征的关系及其表达水平间的相关性研究。方法:收集50例结直肠腺癌组织及癌旁正常组织,用半定量PCR技术检测fbxl20基因及set基因的表达水平并进行分析。结果:fbxl20基因及set基因在结直肠腺癌组织中的表达水平均显著高于癌旁组织;fbxl20基因与set基因在本实验50例结直肠腺癌组织中的表达水平无相关性。但是,在低分化肿瘤组织及D期肿瘤组织中,fbxl20基因与set基因的表达水平呈正相关。结论:fbxl20基因及set基因在结直肠腺癌组织中的表达水平显著升高,其可能与癌细胞的分化过程有关。Objective：To study the relationship between the fbxl20 gene, set gene and the clinocopathologic features, respectively, and do correlation research about the fbxl20 gene and the set gene. Methods ：The mRNA expression level of fbxl20 gene and set gene in 50 pairs of human colorectal adenocarcinoma tissues and matched normal tissues were detected by RT-PCR. Results：The mRNA expres- sion level of fbxl20 gene and set gene in tumor tissues were significantly increased than that in normal tissues. It was not found that the correlation between the mRNA expression level of fbxl20 and the set gene in the 50 tumor tissues. However,the set expressed level was positively correlated with the fbxl20 expressed level in the lower differentiation degree and the Duke＇s D tumor tissues. Conclusion ：The increased mRNA expression level of fbxl20 gene and set gene in tumor tissues was largely related with the process of the cell differentia- tion.

关 键 词：fbxl20 SET 结直肠腺癌 相关性分析 

分 类 号：R735.3[医药卫生—肿瘤]