高效液相色谱法测定虾仁和牡蛎中嘌呤类物质  

Determination of purine in shrimp and oyster by high performance liquid chromatography

作　　者：程庆红[1] 张颖[1] 孟凡艳[2] 王若男[1] 

机构地区：[1]抚顺职业技术学院化工系,辽宁抚顺113006 [2]湖南宝山有色金属矿业有限责任公司质检质量部,湖南郴州424402

出　　处：《应用化工》2013年第10期1923-1926,共4页Applied Chemical Industry

摘　　要：以甲醇-水为流动相,高效液相色谱-紫外检测器测定虾仁和牡蛎提取液中次黄嘌呤、黄嘌呤、腺嘌呤含量,对样品提取和高效液相色谱条件进行优化。结果表明,次黄嘌呤、黄嘌呤、腺嘌呤标准品的检出限分别为3.7×10-9,19×10-9,5.2×10-9g/mL,样品分析日内测定的相对标准偏差分别小于3.2%,3.0%,5.4%,平均加标回收率为80.1%～100.7%。Hypoxanthine,xanthine and adenine in some animal drug materials were determined by means of HPLC with methanol/water as the mobile phase and a UV detector. This process of sample preparation was improved and the conditions of HPLC were also optimized. The results showed that the detection limits of hypoxanthine, xanthine and adenine were 3.7 × 10 - 9,19 × 10 -9,5.2 × 10 -9 g/mL, respectively. The intra-day precisions （RSD） of hypoxanthine, xanthine and adenine were less than 3.2%, 3.0%, 5.4%, respectively. The recovery rate of the samples with added specimen was in a range of 80.1% to 100.7%.

关 键 词：高效液相色谱法 虾仁 牡蛎 嘌呤 

分 类 号：TQ464.4[化学工程—制药化工]