Genetic Diversity and Clustering Analysis of 48Cultivars of Olea euyopaea L.  

48个油橄榄品种的遗传多样性及聚类分析(英文)

作　　者：宁德鲁[1] 陈少瑜[2] 陈海云[1] 李瑞[2] 李勇杰[1] 毛云玲[2] 吴涛[2] 

机构地区：[1]云南省林业科学院,云南昆明650201 [2]云南省林业科学院/云南省森林植物培育与开发利用重点实验室/国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,云南昆明650201

出　　处：《Agricultural Science & Technology》2013年第9期1215-1219,共5页农业科学与技术（英文版）

基　　金：Supported by Key Project of New Product Development in Yunnan Province(2009BB006)~~

摘　　要：Inter-simple sequence repeat（ISSR） molecular markers were applied to analyze the genetic diversity and clustering of 48 introduced and bred cultivars of Olea euyopaea L. Totally 106 DNA bands were amplified by 11 screened primers, including 99 polymorphic bands; the percentage of polymorphic loci was 93.40%, indicating a rich genetic diversity in Olea euyopaea L. germplasm resources. Based on Nei＇s genetic distances between various cultivars, a dendrogram of 48 cultivars of Olea euyopaea L. was constructed using unweighted pair-group（UPMGA）method,which showed that 48 cultivars were clustered into four main categories; 84.6% of native cultivars were clustered into two categories; most of introduced cultivars were clustered based on their sources and main usages but not on their geographic origins. This study will provide references for the utilization and further genetic improvement of Olea euyopaea L. germplasm resources.采用ISSR分子标记技术对48个引种和选育的油橄榄品种进行遗传多样性和聚类分析。11条筛选出的引物共扩增出106条DNA谱带,其中有99条为多态性条带,多态位点百分率为93.40%。数据表明所分析的油橄榄种质具有丰富的遗传多样性。根据各品种的Nei’s遗传距离,运用UPGMA方法构建了所有品种的聚类图,结果 48个油橄榄品种被聚成了4个大类;84.6%的国内选育品种聚于2个类群中;多数引种品种并没有按照地理起源而是依据引种材料来源地和主要用途聚类。研究结果为油橄榄种质资源的利用以及进一步的良种选育提供参考。

关 键 词：Olea euyopaea L. Genetic diversity Clustering analysis 

分 类 号：S565.7[农业科学—作物学]