高效液相色谱法测定人血清中的苄氟噻嗪、环戊噻嗪  

Determination of Bendroflumethiazide and Cyclopenthiazide in Human Plasma by HPLC

作　　者：吴常有 刘佩佩[2] 王翠霞[2] 张兴梅[2] 张强[2] 

机构地区：[1]大庆中蓝石化有限公司,黑龙江大庆163713 [2]烟台大学化学化工学院,山东烟台264005

出　　处：《广州化工》2013年第19期93-94,111,共3页GuangZhou Chemical Industry

摘　　要：以乙醇-K2HPO4-水双水相体系进行样品前处理结合高效液相色谱法分别提取测定了人血清中苄氟噻嗪和环戊噻嗪。试验采用80%(V∶V)乙醇水溶液为萃取液,然后加入适量K2HPO4形成双水相,样品中的亲水性基质保留在双水相下相,而苄氟噻嗪和环戊噻嗪等目标分析物质进入双水相上相,实现了药物残留的高效、快速萃取。双水相上相可直接供HPLC法测定。本方法测定苄氟噻嗪和环戊噻嗪的检出限分别为0.0087μg·mL-1与0.0089μg·mL-1。加标回收率均大于93%。Based on a high efficiency sample pretreatment procedure of aqueous two - phase system （ATPS） of ethanol -K2HPO4 -H2 O, a novel method was developed for analyzing bendroflumethiazide and cyclopenthiazide in human serum coupled with high performance liquid chromatography（HPLC）. Using 80% （V ： V） ethanol -water as extraction agent, after adding with appropriate amount of K2HPO4, the most of hydrophilic matrix were distributed in lower phase, and the drugs （ bendroflumethiazide and cyclopenthiazide） were extracted in upper phase with high extraction efficiencles. The upper phase was direct analysis by HPLC. This method was used for the determination of bendroflumethiazide and cyclopenthiazide in human serum, with the limits of detection were 0. 0087 μg · mL-1 and 0. 0089μg · mL-1, respectively. The spiked recoveries were greater than 93%.

关 键 词：苄氟噻嗪 环戊噻嗪 人血清 双水相 乙醇 HPLC法 

分 类 号：O657.72[理学—分析化学]