人脐带间充质干细胞对NOD/SCID小鼠乳腺原位移植人乳腺癌MCF-7细胞增殖分化的影响  

Influence of Umbilical Cord Mesenchymal Stem Cells on MCF-7 Cells in the Orthotopic Model of NOD/SCID Mice

作　　者：吴晓东[1] 杨永朝 宋献美[3] 

机构地区：[1]郑州市儿童医院,河南郑州450053 [2]阳谷县人民医院,山东阳谷252300 [3]河南职工医学院,河南郑州451191

出　　处：《肿瘤基础与临床》2013年第5期379-382,共4页journal of basic and clinical oncology

摘　　要：目的在NOD/SCID小鼠乳腺原位移植人乳腺癌MCF-7细胞的模型中,研究人脐带间充质干细胞(MSCs)对人乳腺癌MCF-7细胞所形成的异种移植物的增殖和分化情况。方法分别在NOD/SCID小鼠乳腺原位移植人乳腺癌MCF-7细胞、第1代人脐带MSCs或两者混合物,然后处死小鼠,剥离所形成的异种移植物,做病理切片并观察异种移植物的组织形态学特征。结果人乳腺癌MCF-7细胞的异种移植物成瘤率低,人脐带MSCs促进异种移植物的形成并分化成具有人乳腺结构样组织学特征。结论人脐带MSCs具有促进人乳腺癌MCF-7细胞在NOD/SCID小鼠乳腺原位形成具有人乳腺样结构的作用。Objective To implant breast cancer cells in an orthotopic site along with human mesenchymal stem cells （MSCs） in NOD/SCID mice, in order to study the growth and differentiation of human breast cancer MCF-7 cells. Methods MCF-7 cells, MSCs or blend of them were injected into mouse mammary fat pad of the fourth nip- pie. The mice were executed, and the tumors were collected and examined for morphology. Results MCF-7 cells were weakly tumorigenic, human MSCs could promote the formation of xenografts and differentiating into the human mammary gland-like structures. Conclusion Human umbilical cord MSCs can promote human breast cancer MCF- 7 cells differentiating into the human mammary gland-like structures.

关 键 词：小鼠 间充质干细胞 乳腺癌 MCF-7细胞 

分 类 号：R737.9[医药卫生—肿瘤] R730.23[医药卫生—临床医学]